BioFact™ Pfu DNA Polymerase with 10 ml dNTP Mix(each 10 ml, 0. 6 ml), 500U

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SKU : PD116-500

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BioFact™ Pfu DNA Polymerase, a product of recombinant type DNA polymerase from Pyrococcus furiosus, is a suitable product for high-fidelity PCR amplification. The error rate of BioFact™ Pfu DNA Polymerase is 12 times lower than BioFact™ S-Taq DNA Polymerase due to its intrinsic 3’ to 5’ exonuclease activity. It produces blunt ended PCR products and it enables blunt cloning directly using BioFact™ All-in PCR Cloning Kit without A-tailing step. Providing optional 5X Band Helper™ helps amplification of difficult PCR caused by High GC content or complex structure of template. 

  • Recombinant type DNA polymerase
  • High fidelity
  • Low error rate
  • Produces blunt ended PCR

FIGURES (Q,C)

DETAILED INSTRUCTIONS

FEATURES

  • Source : Pyrococcus furiosus
  • 5‘ → 3’ exonuclease activity : No
  • 3‘ → 5’ exonuclease activity : Yes
  • Amplification size : < 3 kb
  • A-tailing : No
  • Error rate : 1-2 bp error/ 106 bp

APPLICATION

  • Routine PCR
  • PCR screening
  • TA cloning
  • Genotyping (SNP, SSR, AFLP, T-RFLP and etc.)

STORAGE TEMPARATURE

  • -20℃ (Stable for 7 days at 25℃)

EXPIRATION DATE

  • 2 years and 3 months
    (in case of storage at -20℃)

 

http://bio-ft.com/en/pro1_1_09

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