PTC3™ (Plant Tissue Culture Contamination Control) is a broad spectrum biostat/fungistat that can be added to plant tissue culture media to reduce microbial and fungal contamination. This product can be added to your media (0.2-2 mL/L) and autoclaved.
Contains the macro- and micronutrients and vitamins as described by Murashige and Skoog (1962) with the following exception: Without Potassium Phosphate Monobasic. Also contains (mg/mL): 300 Sodium Phosphate Monobasic, 150 Adenine Hemisulfate, 1000 Casein, and Ferric Sodium EDTA in place of Ferrous Sulfate and Disodium EDTA.
Contains the macro- and micronutrients as described by Chu (1975). Chu (N6) Medium was developed to promote the initiation, growth, and differentiation of callus from rice pollen cultures.
2,4-Dichlorophenoxyacetic acid being an auxin can support embryogenesis and callus formation at low concentrations, and have herbicidal activity at high concentrations, 2,4-D
Thidiazuron was originally used as a defoliant but this phenyl-urea also has cytokinin activity ,TDZ ,1-Phenyl-3-(1,2,3-thidiazol-5-yl)urea
trans-Zeatin is generally considered to be the most potent of all of the cytokinins. It is also adenine-based, (2E)-2-methyl-4-(1H-purin-6-ylamino)-2-buten-1-ol, 6-(4-Hydroxy-3-methylbut-2-enylamino)purine
Transparency (Clarity): ≥ 80%. Do not use with media containing high levels of calcium such as DKW and Quoirin & Lepoivre. Also if divalent cation (Ca2+, Mg2+) concentrations are below that of 1/2X MS (e.g., our Orchid Maintenance Medium with Charcoal and without Agar) you may need to increase the g/L to obtain free-standing gels. This gelling agent requires melting at 121°C. If your autoclave doesn't reach that temperature for 15-20 min the final gel may appear clumpy. Synonyms: Culturegel™ Type I; Biotech Grade
Ampicillin sodium is a member of the extended-spectrum β-lactam family and similar in structure to penicillin. It is used in the selection of antibiotic cell hybrids and has a high rate of action against many gram-negative bacterial strains.
Carbenicillin is a member of the penicillin β-lactam antibiotics. However, unlike most β-lactams, carbenicillin disodium is limited to primarily gram-negative bacteria including Pseudomonas aeruginosa and common enteric species.
G-418 sulfate, also known as geneticin, is an aminoglycoside antibiotic similar in structure to gentamicin B1. It is commonly used in plant and mammalian cell culture as a selectable marker. It can be used to select for cells transformed with an iNOS promoter construct or neomycin resistance gene.
IPTG is an analog of galactose that is non-metabolizable and inactivates the lac repressor to induce synthesis of β-galactosidase in E. coli. The expression of cloned genes under the control of the lac operon is induced by IPTG. It is also a substrate for thigalactoside transacetylase and has been reported to induce penicillinase in bacteria.
5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside is most commonly known as X-Gal. X-gal is a histochemical substrate for β-galactosidase which cleaves X-Gal to yield an insoluble blue precipitate.
X-Gluc is a chromogenic substrate for beta-glucuronidase (GUS) which is encoded by the gusA (uid A) gene, a widely used reporter gene.
BioFact™ S-Taq DNA Polymerase, one of the most useful in general PCR, is a thermostable DNA polymerase from Thermus aquaticus that possesses 5' to 3' polymerase activity through the unique BioFact’s high purification process.
BioFact™ Genomic DNA Prep Kit has been upgraded to ver 2.0. Through this upgrade, yield has been higher and the step has been reduced than previous version. In addition, simple preparatation can be available through easier protocol than previous kit.
BioFact™ Gel & PCR Purification System is a spin column typed kit which has dual functions of gel extraction and PCR purification.
BioFACT™ Total RNA Prep Kit (Ver.2.0) is intended for isolation of high quality RNA from various samples using glass microfiber membrane as a spin column type.