SARS-CoV-2 PLpro/Papain-Like Protease-His Recombinant Protein

SARS-CoV-2 PLpro/Papain-Like Protease-His Recombinant Protein

 

 

Product Name
SARS-CoV-2 PLpro/papain-like protease-His Recombinant Protein

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SKU/Catalog Number
RCOV04

Size
5ug, 10ug, 20ug, 50ug

Form
Liquid

Description
Recombinant 2019-nCoV PLpro/papain-like protease, part of a large replicase polyprotein 1ab (E1564-Y1882), was expressed in E. coli cells with an N-terminal His tag. The gene accession number of polyprotein 1ab is QHD43415.

Storage & Handling
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Cite This Product
SARS-CoV-2 PLpro/papain-like protease-His Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # RCOV04)

Formulation
Recombinant protein stored in 50mM sodium phosphate, pH 7.5, 300mM NaCl, 150mM imidazole, 1mM DTT, 10% glycerol.

2019-nCoV papain-like protease (PLpro), also known as SARS-CoV-2 PLpro, is a virally encoded cysteine protease that acts on three cleavage sites of the viral polyprotein to release mature non-structural proteins (nsp) 1, 2 and 3, a process that is essential for viral replication. In addition, PLpro possesses deubiquitinating and deISGylating activities, thus negatively regulating the ubiquitin and ISG15-dependent host immune response during viral infection. PLpro also participates together with nsp4 in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication.

 

 

 

https://www.bosterbio.com/sars-cov-2-plpro-papain-like-protease-his-recombinant-protein-rcov04-boster.html