Cell Counting Kit-8 （CCK-8）
Price: 100tests = 1,500 THB (Exc VAT7%)
500tests = 3,500 THB (Exc VAT7%)
Application notes: Cell Counting Kit-8 (CCK-8) is designed to detect cell proliferation and cell toxicity based on WST-8 is reduced by dehydrogenases in cells to give an orange colored product (formazan). The amount of the formazan in cells is directly proportional to the number of living cells. Tests confirm that performance equals or exceeds popular CCK-8 kit from other suppliers.
Formulation: Liquid solution
Storage buffer: Liquid in PBS, pH 7.4, containing 0.02% sodium azide as preservative and 50% Glycerol.
Storage instructions: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping: Gel pack with blue ice.
Precautions: • Since the CCK-8 assay is based on the dehydrogenase activity detection in viable cells, conditions or chemicals that affect dehydrogenase activity in viable cells may cause discrepancy between the actual viable cell number and the cell number determined using the CCK-8 assay.
• Be careful not to introduce bubbles to the wells, since they interfere with the O.D. reading.
• The incubation time varies by the type and number of cells in a well. Generally, leukocytes give weak coloration, thus a long incubation time (up to 4 hours) or a large number of cells (~105 cells/well) may be necessary.
• If the color or PH of culture media is changed due to long-time culture, please change the culture media when adding CCK-8.
Background: Cell Counting Kit-8 (CCK-8) utilizes highly water-soluble tetrazolium salt WST-8:[2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt] changed to a water-soluble orange formazan dye by reduction. CCK-8 is low toxicity and allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation and cytotoxicity assays. The amount of the formazan dye generated by dehydrogenases in cells is directly proportional to the number of living cells.
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