• 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel,
• 1.5~2.5 μl per well for general Western transferring.
• Apply more for thicker (>1.5 mm) or larger gel.
The BLUEstainTM 2 Protein ladder is a three-color protein standard with 13 prestained proteins, covering a wide range molecular weights from 3.5 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).
The BLUEstainTM 2 Protein ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute or add reducing agent before loading.
Storage/Handling: Store at room temperature for 3 months. Store at -20°C for 24 months.
This product is stable for shipping at ambient temperatures.
Contents: Approximately 0.1~0.4 mg/ml of each protein in the bufffer (20mM Tris-phosphate, pH 7.5 at 25°C), 2% SDS, 10mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol).
Carbenicillin is a member of the penicillin β-lactam antibiotics. However, unlike most β-lactams, carbenicillin disodium is limited to primarily gram-negative bacteria including Pseudomonas aeruginosa and common enteric species.
Cefotaxime is a third generation, broad-spectrum cephalosporin antibiotic effective against various gram-negative and gram-positive bacteria. However, unlike many other cephalosporins, cefotaxime is not effective against Pseudomonas aeruginosa bacteria.
Ampicillin sodium is a member of the extended-spectrum β-lactam family and similar in structure to penicillin. It is used in the selection of antibiotic cell hybrids and has a high rate of action against many gram-negative bacterial strains.