SKU : MB-T0637
หมวดหมู่ : 12. Lab Equipment , MB Cell ,
แบรนด์ : MB cell
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TSB (Tryptic Soy Broth) Hemin Menadione [MB-T0637]
Medium used for the cultivation and enumeration of anaerobic bacteria.
PROCEDURE
Suspend 25.0 G of powder in 950 mL of distilled or deionized water. Heat to boiling until completely dissolved. Sterilized by autoclave at 121°C for 15 minutes. Cool to 45 - 50°C in water bath. Aseptically add 5% of Sheep Blood Defibrinated (MB-S1876) and 2 vials of TSA Hemin Menadione A supplement (MB-T0707) and 2 vials of TSA Hemin Menadione B supplement (MB-T0708). Mix well. Pour into tubes.
STORE
The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared medium at 2 - 8°C.
