G-418 sulfate, also known as geneticin, is an aminoglycoside antibiotic similar in structure to gentamicin B1. It is commonly used in plant and mammalian cell culture as a selectable marker. It can be used to select for cells transformed with an iNOS promoter construct or neomycin resistance gene.
IPTG is an analog of galactose that is non-metabolizable and inactivates the lac repressor to induce synthesis of β-galactosidase in E. coli. The expression of cloned genes under the control of the lac operon is induced by IPTG. It is also a substrate for thigalactoside transacetylase and has been reported to induce penicillinase in bacteria.
X-Gluc is a chromogenic substrate for beta-glucuronidase (GUS) which is encoded by the gusA (uid A) gene, a widely used reporter gene.
BioFact™ S-Taq DNA Polymerase, one of the most useful in general PCR, is a thermostable DNA polymerase from Thermus aquaticus that possesses 5' to 3' polymerase activity through the unique BioFact’s high purification process.
BioFact™ Genomic DNA Prep Kit has been upgraded to ver 2.0. Through this upgrade, yield has been higher and the step has been reduced than previous version. In addition, simple preparatation can be available through easier protocol than previous kit.
BioFact™ Gel & PCR Purification System is a spin column typed kit which has dual functions of gel extraction and PCR purification.
BioFACT™ Total RNA Prep Kit (Ver.2.0) is intended for isolation of high quality RNA from various samples using glass microfiber membrane as a spin column type.