McCoy's 5A (modified) medium, GlutaPlus, HEPES, 500 mL

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SKU : G4541-500ML

แบรนด์ : Servicebio

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Product Information

Product Name

Cat. No.

Spec.

McCoy's 5A (Modified) Medium, GlutaPlus, HEPES

G4541-500ML

500 mL

 


Product Description

McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues. McCoy's 5A modified contains a high concentration of D-glucose, bacterial peptone and reduced glutathione compared to other media.

This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Add 5-10% serum or serum-free additives depending on the cell type.

This product is sterilized by 0.1μm filter membrane, pH 7.0-7.4, contains 16.67 mM D-glucose, 1.5 mM L-Alanyl-L-Glutamine, 25 mM HEPES buffer system, phenol red indicator, no sodium pyruvate.

HEPES is an excellent biological buffering agent with no toxic effect on cells, and the medium supplemented with HEPES can maintain a constant pH range for a long time.

 


Storage and Shipping Conditions

Ship at room temperature; Store away from light at 2-8, valid for 12 months.

 


Note

1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.

2. For best results, do not freeze and thaw repeatedly.

3. For your safety and health, please wear safety glasses, gloves and protective clothing.

 

For Research Use Only!

G4541 McCoy's 5A, modified, HEPES 

Components

Concentration


Components

Concentration

mg/L

mM

mg/L

mM

Amino acids




Thiamine hydrochloride

0.20

0.000593472

Glycine

7.5

0.1000


Vitamin B12

2.00

0.001476015

L-Alanine

13.9

0.156


i-Inositol

36.0

0.2000

L-Arginine hydrochloride

42.1

0.1995


Inorganic salts



L-Asparagine monohydrate

51.14

0.3409


Calcium Chloride (CaCl2)(anhyd.)

100

0.901

L-Asparagine

19.97

0.1502


Magnesium sulfate(MgSO4)(anhyd.)

200

0.813

L-Cysteine hydrochloride hydrate

31.5

0.2603


Potassium Chloride (KCl)

400

5.333

L-Glutamic acid

22.1

0.1503


Sodium bicarbonate(NaHCO3)

2200

26.190

L-Alanyl-L-Glutamine

326.1

1.5014


Sodium chloride (NaCl)

5100

87.931

L-Histidine hydrochloride monohydrate

20.96

0.0998


Sodium Phosphate monobasic(NaH2PO4)(anhyd.)

504

4.203

L-Isoleucine

19.7

0.1504


Other components



L-Leucine

39.36

0.3005


Peptone

600

Infinity

L-Lysinehydrochloride

39.36

0.3005


D-Glucose (Dextrose)

3000

16.67

L-Lysine Hydrochloride

36.5

0.1995


Reduced glutathione

0.5

0.0016

L-Methionine

14.9

0.1000


Phenol Red

9.4

0.0266

L-Phenylalanine

16.5

0.1000


HEPES

5958

25

L-Proline

17.3

0.1504





L-Serine

26.3

0.2505





L-Threonine

17.9

0.1504





L-Tryptophan

3.06

0.0152





L-Tyrosine disodium salt

26.1

0.1000





L-Valine

17.6

0.1504





Vitamins







L-Ascorbic acid

0.50

0.002840909





Biotin

0.20

0.000819672





Choline chloride

5.00

0.035714287





D-Calcium pantothenate

0.20

0.000419287





Folic Acid

10.00

0.022675738





Niacinamide

0.50

0.00409836





Nicotinic acid

0.50

0.004065041





Para-Aminobenzoic Acid

1.00

0.00729927





Pyridoxal hydrochloride

0.50

0.002450981





Pyridoxine hydrochloride

0.50

0.002427185





Riboflavin

0.20

0.000531915





 

 

 

 

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