Products
Cell Biology Reagents | Consumables
Cell Culture Media
DMEM/F-12
SKU : G4612-500ML
หมวดหมู่ : 2. Cell & Molecular Biology , Cell Culture Medium / Sera , Servicebio ,
แบรนด์ : Servicebio
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Product Information
Product Name | Cat. No. | Spec. |
DMEM/F-12, GlutaPlus, HEPES | G4612-500ML | 500 mL |
Product Description/Introduction
DMEM/F-12 medium was modified by mixing DMEM with Ham's F-12 medium at a ratio of 1:1. The modified DMEM/F-12 medium is richer in nutrients and contains more micronutrients, which is a widely used basal medium for supporting the growth of many different mammalian cells, as well as for the culture of mammalian cells at low serum levels and for clonal density culture. DMEM/F-12 has also been used as a base medium for the development of serum-free media.
This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Add 5-10% serum or serum-free additives depending on the cell type.
This product is sterilized by 0.1μm filter membrane, pH 7.0-7.4, contains 17.5 mM D-glucose, 2.5 mM L-Alanyl-L-Glutamine, 0.5 mM sodium pyruvate, phenol red indicator and HEPES buffer system.
HEPES is a commonly used biological buffering agent, which can keep the medium at a constant pH range for a long time and effectively prevent the adverse effects of large pH fluctuations on cell growth.
Storage and Shipping Conditions
Ship at room temperature; Store away from light at 2-8, valid for 12 months.
Note
1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.
2. For best results, do not freeze and thaw repeatedly.
3. For your safety and health, please wear safety glasses, gloves and protective clothing.
For Research Use Only!
G4612 DMEM/F-12 Formula
Components | Concentration | Components | Concentration | |||
mg/L | mM | mg/L | mM | |||
Amino acids | Inorganic salts | |||||
Glycine | 18.75 | 0.250 | Calcium Chloride (CaCl2)(anhyd.) | 116.6 | 1.050 | |
L-Alanine | 4.45 | 0.050 | Cupric Sulfate (CuSO4·5H2O) | 0.0013 | 0.0000052 | |
L-Arginine monohydrochloride | 147.5 | 0.699 | Ferric Nitrate (Fe(NO3)3·9H2O) | 0.05 | 0.0001238 | |
L-Asparagine monohydrate | 7.5 | 0.050 | Ferrous Sulfate (FeSO4·7H2O) | 0.417 | 0.0015 | |
L-Asparagine | 6.65 | 0.050 | Magnesium chloride (MgCl2)(anhyd.) | 28.64 | 0.301 | |
L-Cysteine hydrochloride hydrate | 17.56 | 0.100 | Magnesium sulfate (MgSO4) (anhyd.) | 48.84 | 0.407 | |
L-Cystine dihydrochloride | 31.29 | 0.100 | Potassium Chloride (KCl) | 311.8 | 4.157 | |
L-Glutamic acid | 7.35 | 0.050 | Sodium bicarbonate(NaHCO3) | 1200 | 14.27 | |
L-Alanyl-L-Glutamine | 543.0 | 2.500 | Sodium chloride (NaCl) | 6995.5 | 120.6 | |
L-Histidine hydrochloride monohydrate | 31.48 | 0.150 | Disodium (hydrogen) phosphate(Na2HPO4)(anhyd.) | 71.02 | 0.500 | |
L-Isoleucine | 54.47 | 0.416 | Sodium dihydrogen phosphate(NaH2PO4)(anhyd.) | 54.34 | 0.453 | |
L-Leucine | 59.05 | 0.451 | Zinc Sulfate(ZnSO4·7H2O) | 0.432 | 0.0015 | |
L-Lysine hydrochloride | 91.25 | 0.499 | Other components | |||
L-Methionine | 17.24 | 0.116 | D-Glucose (Dextrose) | 3151 | 17.51 | |
L-Phenylalanine | 35.48 | 0.215 | Hypoxanthine Na | 2.39 | 0.015 | |
L-Proline | 17.25 | 0.150 | Linoleic Acid | 0.042 | 0.00015 | |
L-Serine | 26.25 | 0.250 | Lipoic acid | 0.105 | 0.00051 | |
L-Threonine | 53.45 | 0.449 | Phenol Red | 8.1 | 0.022 | |
L-Tryptophan | 9.02 | 0.044 | Putrescine dihydrochloride | 0.081 | 0.00050 | |
L-Tyrosine disodium salt dihydrate | 55.79 | 0.214 | Thymidine | 0.365 | 0.00151 | |
L-Valine | 52.85 | 0.452 | Sodium pyruvate | 55 | 0.500 | |
Vitamins | HEPES | 3574.5 | 15.0 | |||
Biotin | 0.0035 | 0.0000143 | ||||
Choline chloride | 8.98 | 0.0641 | ||||
D-Calcium pantothenate | 2.24 | 0.0047 | ||||
Folic Acid | 2.65 | 0.0060 | ||||
Niacinamide | 2.02 | 0.0166 | ||||
Pyridoxine hydrochloride | 2.013 | 0.0098 | ||||
Riboflavin | 0.219 | 0.0006 | ||||
Thiamine hydrochloride | 2.17 | 0.0064 | ||||
Vitamin B12 | 0.68 | 0.0005 | ||||
i-Inositol | 12.6 | 0.07 | ||||
