SKU : G4560-500ML
หมวดหมู่ : 2. Cell & Molecular Biology , Cell Culture Medium / Sera , Servicebio ,
แบรนด์ : Servicebio
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Product Information
Product Name | Cat. No. | Spec. |
Hams F-12K, GlutaPlus | G4560-500ML | 500 mL |
Product Description/Introduction
Ham's F-12K medium is a Kaighn's improved version of Ham's F-12 nutrient mix, originally designed for culturing primary human hepatocytes and differentiated mouse and chicken cells under low serum conditions. Ham's F-12K improves the amino acid and sodium pyruvate concentration, reduces the glucose content, and modifies the composition and content of inorganic salts based on the original Ham's F-12.Compared to other medium, Ham's F-12K contains many unique components such as putrescine, thymine, hypoxanthine, and zinc, in addition to a higher content of sodium pyruvate.
This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Need to add serum or serum-free additives depending on the cell type.
This product is filtered and sterilized by 0.1 μm filter membrane, pH 7.0-7.4, contains 7.0 mM D-Glucose, 2.0 mM L-Alanyl-L-Glutamine, 2.0 mM sodium pyruvate and phenol red indicator, without HEPES.
Storage and Shipping Conditions
Ship at room temperature; Store away from light at 2-8, valid for 12 months.
Note
1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.
2. For best results, do not freeze and thaw repeatedly.
3. For your safety and health, please wear safety glasses, gloves and protective clothing.
For Research Use Only!
G4560 Hams F-12K Formula
Components | Concentration | Components | Concentration | |||
mg/L | mM | mg/L | mM | |||
Amino acids | Inorganic salts | |||||
Glycine | 15 | 0.2 | Calcium Chloride (CaCl2)(anhyd.) | 102 | 0.9189 | |
L-Alanine | 18 | 0.2022 | Copper Sulfate Pentahydrate (CuSO4·5H2O） | 0.002 | 0.0000080 | |
L-Arginine hydrochloride | 422 | 2.0 | Iron Sulfate Heptahydrate (FeSO4·5H2O） | 0.8 | 0.002878 | |
L-Asparagine-H2O | 30 | 0.2 | Magnesium Chloride (MgCl2)(anhyd.) | 49.7 | 0.5231 | |
L-Aspartic Acid | 26.6 | 0.2 | Magnesium Sulfate (MgSO4) (anhyd.) | 192 | 1.600 | |
L-Cysteine hydrochloride-H2O | 70 | 0.3977 | Potassium Chloride (KCl) | 285 | 3.8000 | |
L-Glutamic Acid | 29 | 0.1973 | Sodium Bicarbonate (NaHCO3) | 2500 | 29.76 | |
L-Alanyl-L-Glutamine | 434.4 | 2 | Sodium Chloride (NaCl) | 7530 | 129.9 | |
L-Histidine hydrochloride-H2O | 45.8 | 0.2181 | Dibasic sodium phosphate (Na2HPO4) (anhyd.) | 115.5 | 0.813 | |
L-Isoleucine | 7.88 | 0.06015 | Sodium dihydrogen Phosphate monobasic (NaH2PO4) (anhyd.) | 59.0 | 0.492 | |
L-Leucine | 26.2 | 0.2 | Zinc Sulfate Heptahydrate (ZnSO4·7H2O） | 0.144 | 0.0005 | |
L-Lysine hydrochloride | 73 | 0.3989 | Other components | |||
L-Methionine | 8.96 | 0.06013 | D-Glucose (Dextrose) | 1260 | 7 | |
L-Phenylalanine | 9.92 | 0.06012 | Hypoxanthine | 4 | 0.03053 | |
L-Proline | 69 | 0.6 | Alphalipoic Acid | 0.21 | 0.001019 | |
L-Serine | 21 | 0.2 | Phenol red | 3 | 0.007970 | |
L-Threonine | 23 | 0.1933 | Diaminobutane dihydrochloride | 0.32 | 0.001987 | |
L-Tryptophan | 4.1 | 0.02010 | Sodium pyruvate | 220 | 2.0 | |
L-Tyrosine Disodium Salt | 13.5 | 0.05153 | Thymine | 0.7 | 0.002892 | |
L-Valine | 23 | 0.1966 | ||||
Vitamins | ||||||
Biotin | 0.07 | 0.0002869 | ||||
Choline chloride | 14 | 0.10 | ||||
D-Calcium pantothenate | 0.5
| 0.001048 | ||||
Folic Acid | 1.3 | 0.002948 | ||||
Niacinamide | 0.037 | 0.0003033 | ||||
Pyridoxine hydrochloride | 0.06 | 0.0002913 | ||||
Riboflavin | 0.04 | 0.0001064 | ||||
Thiamine hydrochloride | 0.3 | 0.0008902 | ||||
Vitamin B12 | 1.4 | 0.001033 | ||||
i-Inositol | 18 | 0.10 | ||||
