DMEM, high glucose, no L-Glutamine, no calcium, 500 mL

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SKU : G4519-500ML

แบรนด์ : Servicebio

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Product Information

Product Name

Cat. No.

Spec.

DMEM, high hlucose, no L-Glutamine, no calcium

G4519-500ML

500 mL




Product Description

Dulbecco's Modified Eagle Medium (DMEM), is a modified medium based on MEM, is now widely used in various cell cultures. DMEM High Glucose Medium is commonly used for fast-growing cells with low adhesion. It can be used for the culture of many kinds of mammalian primary cells, such as primary fibroblasts, neuronal cells, glial cells, HUVEC, smooth muscle cells, etc. It can also be used for the culture of some cell lines, such as Hela, 293, Cos-7 and PC-12.

This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients required for cell culture, does not contain protein or growth factors, need to be added according to the type of cells 5-10% serum or serum-free additives to use.

This product is filtered and sterilized by 0.1 μm filter membrane, pH 7.0-7.4, containing 25 mM D-glucose, with phenol red indicator, calcium ion, L-glutamine, sodium pyruvate have not been added, and does not contain HEPES buffer system..




Storage and Shipping Conditions

Ship at room temperature; Store away from light at 2-8, valid for 12 months.




Note

1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.

2. Avoid freezing the culture medium. Freezing may cause precipitation and precipitation of nutrients, which may not be completely dissolved after rewarming, affecting the cell culture effect. In addition, when the medium is stored at 2-8, do not store it completely close to the inner wall of the refrigerator to prevent the medium from freezing due to localized low temperature.

3. For your safety and health, please wear safety glasses, gloves or protective clothing.

 

For Research Use Only!

G4519 DMEM (High Glucose) Medium Formula

Components

Concentration


Components

Concentration

mg/L

mM

mg/L

mM

Amino acids




Inorganic salts



Glycine

30

0.4


Ferric nitrate nonahydrate ( Fe(NO3)39H2O)

0.1

0.0002

L-Arginine hydrochloride

84

0.4


Magnesium Sulfate (MgSO4) (anhyd.)

97.67

0.80

L-Cystine 2HCl

62.6

0.2


Potassium Chloride (KCl)

400

5.36

L-Histidine hydrochloride-H2O

42

0.2


Sodium Bicarbonate (NaHCO3)

3700

44.04

L-Isoleucine

105

0.8


Sodium Chloride (NaCl)

6400

109.50

L-Leucine

105

0.8


Sodium Phosphate monobasic (NaH2PO4) (anhyd.)

108.7

0.906

L-Lysine hydrochloride

146

0.8


Other components



L-Methionine

30

0.2


D-Glucose (Dextrose)

4500

24.98

L-Phenylalanine

66

0.4


Phenol Red

14.95

0.0422

L-Serine

42

0.4





L-Threonine

95

0.8





L-Tryptophan

16

0.08





L-Tyrosine disodium salt dihydrate dihydrate

103.79

0.4





L-Valine

94

0.8





Vitamins







Choline chloride

4.0

0.0286





D-Calcium pantothenate

4.0

0.0084





Folic Acid

4.0

0.0091





Niacinamide

4.0

0.0328





Pyridoxine hydrochloride

4.0

0.0195





Riboflavin

0.4

0.0011





Thiamine hydrochloride

4.0

0.0119





Meso-Inositol

7.2

0.0389












 

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