Recombinant Human CD354 Protein (His-tag)

Gene Name Synonym

TREM1, CD354, TREM-1

Species

Human

Accession

Q9NP99

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Ala21-Asn205 is expressed with a 10×His tag at the C terminus

Molecular Weight

20.2 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

CD354, Triggering Receptor of Myeloid Cells-1(TREM-1), is a potent amplifier of myeloid immune responses. CD354 is a type I transmembrane protein with a single Ig-like domain, and is selectively expressed on blood neutrophils and a subset of monocytes.TREM-1 potently amplified the function of TLR and Nod-like receptors (NLR) which resulted in enhanced cytokine production. TREM-1 was first described as a costimulatory receptor on myeloid cells. Antagonizing TREM-1 with antibodies, fusion proteins, or inhibitory peptides reduces inflammation in experimental arthritis and colitis models.TREM-1 mRNA and protein were elevated in inflammatory bowel disease, experimental colitis, inflammatory arthritis, and other noninfectious inflammatory diseases.TREM1 performs a critical function in immune responses involved in host defense against microbial challenges, and is suggested to be a potential therapeutic target for septic shock.

Data

SDS-PAGE

Bioactivity

Testing in progress

Recombinant Human CD354 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 35-60 kDa.