Taq DNA Polymerase, 1000 U

Product Information

Product Description/Introduction

This product Taq DNA polymerase is a mutant Taq DNA polymerase obtained through in vitro evolutionary screening based on wild-type Taq DNA polymerase. It has polymerization activity of 5'-3' and exonuclease activity of 5'-3', but no exonuclease activity of 3'-5'. The amplification rate is 1 min/kb, and the amplification product has an "A" base at the 3 'end, which can effectively amplify DNA fragments within 7 kb.

Source: E.coli cells with a cloned polA gene from Thermus aquaticusi.

Enzyme activity definition: Using activated salmon sperm DNA as template primer, the enzyme required for incorporation of 10nmol deoxynucleotides into acid insoluble substances was defined as an enzyme activity unit within 30min at 74.

Purity and Concentration: 95% by SDS-PAGE;endogenous nucleic acid < 1 pg/μL detected by qPCR; 5 U/μL.

Enzyme storage buffer: 10 mM Tris-HCl，100 mM KCl，1 mM DTT，0.1 mM EDTA，0.5% Tween 20，50% Glycerol，pH 7.4；

Storage and Shipping Conditions

wetice transport; Store at -20 for 12 months.

Product Content

Assay Protocol / Procedures

1. This product is mainly sold in the form of Taq DNA polymerase raw materials, and customers need to optimize the reaction buffer and reaction system according to their own needs. G3302 is recommended for purchase of PCR premixes.

Note

1. The optimization of PCR reaction can be adjusted from the aspects of Mg2+concentration, template amount, enzyme amount, annealing temperature, etc.

2. Please configure the reaction system on ice, as Taq DNA polymerase also has some reactivity at room temperature.

3. For your safty and health,please wear safety glasses, gloves, or protective clothing.

For Research Use Only!

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