Crystal violet stain, 50 mL (for Nucleic acid staining)

Product Information

Description

Crystal violet, also known as Gentian violet, is an alkaline dye that binds to the DNA in the cell nucleus to stain the nucleus.

The concentration of our Crystal Violet Dye is 0.1%, which can be used to stain cells, tissue sections and bacteria to observe cell morphology.

Storage and Handling Conditions

Ship and store at room temperature away from light, valid for is 12 months.

Component

Protocol

1. For adherent cells: cells were fixed with 4% paraformaldehyde (recommended G1101) for 10-15 min and washed with water for 3 times, 5 min each. Crystal violet solution was dropped to cover the cells, and the cells were stained at room temperature for 3-10 min (the time was adjusted according to the staining results and requirements). After being fully washed with tap water, the cells could be observed under the microscope.

2. For suspended cells: suspended cells were stained with crystal violet solution in a ratio of 10:1 for 3-10min (the time was adjusted according to the staining results and requirements), and observed under microscope after directly dropped on the slides.

3. For paraffin sections of tissues: the sections were dewaxed and rehydrated, and crystal violet solution was added to cover the tissues, stained at room temperature for 3-10 min (the time was adjusted according to the staining results and requirements). After being fully washed with tap water, the sections could be observed under the microscope.

Note

1. 50 mL of solution can be used to stain approximately 50 sections (drop staining).

2. For your safety and health, please wear lab coat and disposable gloves during operation.

For Research Use Only!

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