Tricine SDS-PAGE Running Buffer (Powder), 1 L

Product Information

Product Introduction

This product Tricine SDS-PAGE Running Buffer (Powder) that is Tricine SDS-PAGE protein gel electrophoresis buffer in the form of powder, this product is a fine powder, rapid dissolution, easy to use, formulated to dissolve for Tricine system protein gel electrophoresis. Tricine gels are designed for the separation of low molecular weight proteins. In this buffer system, Tricine is used instead of glycine in the electrophoresis buffer to separate low molecular weight proteins more efficiently and improve the resolution of small molecular weight peptides. The main components of the product are 100 mM Tris, 100 mM Tricine, 0.1% SDS, pH 8.2-8.5@25.

Storage and Shipping Conditions

Ship and store at room temperature away from light, valid for 36 months.

Assay Procedures

1. Each package of powder should be dissolved in 1 L of pure water and stir until dissolved.

2. The electrophoresis voltage can be 150 V throughout, the electrophoresis time is about 60 min, and the whole process requires ice bath electrophoresis.

Note

1. Do not mix with other electrophoresis buffer, this product just for denaturing electrophoresis in Tricine gels.

2. This electrophoresis solution is not recommended for reuse.

3. The electrophoretic voltage should not be too high, and electrophoretic heat production may result in unfavorable separation of small molecule proteins.

4. For your safety and health, please wear appropriate protective clothing and gloves.

For research use only!

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