Tetracyclines ELISA Test Kit

Brand: FSTest
CatNO: DR32.5072.5
Specifications: 96tests/kit
Shelf life: 12 months
Application: Designed for quantitative detection of tetracycline-class antibiotics (including tetracycline, chlortetracycline, and oxytetracycline) in animal tissues, seafood, honey, and milk, ensuring food safety and regulatory compliance.

FSTest Tetracyclines ELISA Test Kit – High-Sensitivity Detection for Banned Antibiotic Residues
Introduction:
The FSTest Tetracyclines ELISA Test Kit is a precise immunoassay designed for quantitative detection of tetracycline-class antibiotics (including tetracycline, chlortetracycline, and oxytetracycline) in animal tissues, seafood, honey, and milk. With a 2ppb detection limit across all matrices, this kit ensures compliance with global regulations prohibiting tetracycline residues in food products.

Key Features & Benefits:
  Comprehensive Detection - Covers 4 key tetracyclines including: Tetracycline (100% cross-reactivity), Oxytetracycline (80%), Chlortetracycline (45%), Doxycycline (10%)
  Ultra-Sensitive - 2ppb detection limit meets strictest global MRLs
  Multi-Matrix Validation - Certified for meat, seafood, dairy, and honey
  Regulatory Ready - Complies with EU/China/USDA zero-tolerance policies
  Efficient Workflow - 96-well format processes 20+ samples per run
  Simplified Protocols - Matrix-specific sample preparation methods

Applications:
Livestock and poultry antibiotic monitoring
Seafood and aquaculture safety testing
Dairy product quality control
Honey export compliance verification
Veterinary drug residue surveillance

Testing Principle:
Competitive ELISA measuring absorbance at 450nm (inversely proportional to tetracycline concentration) against a 5-point standard curve (0-2.7ppb).

How to Use:

1. Sample Preparation:
   Meat/Seafood: HCl extraction + pH adjustment
   Honey: Simple water dilution
   Milk: Trichloroacetic acid precipitation
   
   
2. Test Procedure:
   Add 50μL sample/standard
   Add 50μL enzyme conjugate & antibody
   Incubate 30min (20-28°C)
   Wash 4-5 times
   Add substrate, incubate 30min
   Add stop solution
   Read at 450nm
   
   
3. Result Interpretation:
   Compare to standard curve (0.1-2.7ppb)
   Apply 10× dilution factor for all matrices

Why Choose FSTest Tetracyclines ELISA Kit?
  Most Regulated Antibiotic Class - Essential for compliance testing
  Simplified Dairy Testing - Special milk protocol with trichloroacetic acid
  Time-Efficient - Complete analysis in<2.5 hours
  Cost-Effective - 96 tests/kit reduces per-sample cost
  Consistent Performance - ≤10% CV ensures reliable results

Technical Specifications:
Detection Limit: 2ppb (all matrices)
LOQ: 0.2ppb
Accuracy: 70-130% recovery
Precision: ≤10% CV
Format: 96-well microplate
Storage: 2-8°C (12 months)

User manual

• DR32.5072.5 Tetracyclines ELISA Test Kit User Manual

https://www.fstestcorp.com/products-detail/id-172.html