OX.2253-PLF Inverted trinocular microscope for fluorescence with mechanical stage PL Fluarex

Inverted Oxion Inverso brightfield, phase contrast and fluorescence series
Inverted trinocular microscope for fluorescence with mechanical stage PL Fluarex 10/20/40x - Rail for 2 filter blocks and BF position, with transportation box

Model : OX.2253-PLF

• Pair HWF 10x/22 mm eyepieces
  
• Trinocular head, 45° inclined tubes. Tubes can be positioned in upper or lower positions
  
• Interpupillary distance 54-75 mm. Diopter adjustment on left eyepiece
  
• Reversed quintuple revolving nosepiece on ball-bearings
  
• Plan Fluarex PL 10x, 20x, S40x IOS objectives.
  
• All optics are anti-fungus treated and anti-reflection coated for maximum light throughput
  250 x 230 mm stage with 120 x 78 mm mechanical stage
  
• Supplied with glass insert with 30 mm hole, a metallic insert with 25 mm hole, a metallic
  insert for 35 mm diameter Petri dish holder, a 76x26 mm slide holder with 54mm Petri dish
  holder and a Multiwell holder with 65 mm Petri dish holder
  
• Coaxial coarse and fine adjustments. Precision 2 μm, 0.2 mm per rotation. Total travel
  approximately 25 mm
  
• Supplied with metallic insert for 35 mm Petri dishes and Terasaki holder.
  
• N.A. 0.30 condenser with iris diaphragm and filter holder, working distance 72 mm
  
• Transmitted 100 W mercury vapor fluorescence and 5 W NeoLED brightfield illumination
  with internal power supply 100-240 V (CE) and UV protection shield
  
• Supplied with 2 filter blocks with standard filter sets:
  - Blue: EX465-495 DM505LP EM515-558,
  - Green: EX500-580 DM600LP EM605-665
  
• Supplied with power cord and transportation box

ACCESSORIES

OX.6010 Wide field eyepiece HWF 10x/22 mm for Oxion Inverso inverted microscopes	OX.6015 Wide field eyepiece HWF 15x/16 mm for Oxion Inverso inverted microscopes	OX.6110 Wide field eyepiece HWF 10x/22 mm with reticle for Oxion Inverso inverted microscopes	OX.6099 Pair of eyecups for Oxion Inverso inverted microscopes
OX.8304 Plan LWD 4x/0.13 IOS objective. Working distance 11 mm	OX.8310 Plan LWD 10x/0.30 IOS objective, corrected for 1.2 mm. Working distance 7.45 mm	OX.8320 Plan LWD 20x/0.45 IOS objective, corrected for 1.2 mm. Working distance 6.92 mm	OX.8340 Plan LWD 40x/0.65 IOS objective, corrected for 1.2 mm. Working distance 2.74 mm
OX.8360 Plan LWD 60x/0.75 IOS objective, corrected for 1.2 mm. Working distance 1.28 mm	OX.8404 Plan phase LWD 4x/0.13 infinity corrected IOS, objective. Working distance 10.75 mm	OX.8410 Plan phase LWD 10x/0.25 infinity corrected IOS, objective. Working distance 7.45 mm	OX.8420 Plan phase LWD 20x/0.40 infinity corrected IOS, objective. Working distance 6.92 mm
OX.8440 Plan phase LWD 40x/0.60 infinity corrected IOS, objective. Working distance 2.74 mm	OX.8604 Plan LWD 4x/0.13 IOS Fluarex objective, corrected for 1.2 mm. Working distance 18.95mm	OX.8610 Plan LWD 10x/0.30 IOS Fluarex objective, corrected for 1.2 mm. Working distance 7.27 mm	OX.8620 Plan LWD 20x/0.45 IOS Fluarex objective, corrected for 1.2 mm. Working distance 6.03 mm
OX.8640 Plan LWD 40x/0.65 IOS Fluarex objective, corrected for 1.2 mm. Working distance 1.79 mm	OX.8660 Plan LWD 60x/0.75 IOS Fluarex objective, corrected for 1.2 mm. Working distance 1.28 mm	OX.8720 Plan LWD Phase contrast 20x/0.40 IOS Fluarex objective, corrected for 1.2 mm. Working distance 6.12 mm	OX.8740 Plan LWD Phase contrast 40x/0.65 IOS Fluarex objective, corrected for 1.2 mm. Working distance 1.79 mm
OX.9148 Telescope for fluorescence alignment for Oxion Inverso inverted microscopes	OX.9149 Telescope for phase contrast alignment for Oxion Inverso inverted microscopes	OX.9161 Slider with 10x/20x and 40x phase contrast annuli for Oxion Inverso inverted microscopes	OX.9190 Slider with 4x/10x and 20x/40x phase contrast annuli for Oxion Inverso inverted microscopes
OX.9500 Mechanical X-Y stage, 120 x 78 mm for Oxion Inverso inverted microscopes	OX.9506 transparent glass insert with hole for Oxion Inverso inverted microscopes	OX.9507 transparent glass insert without hole for Oxion Inverso inverted microscopes	OX.9508 Metal insert with hole for Oxion Inverso inverted microscopes
OX.9510 Metal insert for microplates (Multiwell) for Oxion Inverso inverted microscopes	OX.9512 Insert with 76 x 26 mm slide holder and diameter 54 mm cell culture dish holder	OX.9535 Petri dish insert 35 mm for Oxion Inverso inverted microscopes	OX.9550 Stage extension plate
OX.9599 Universal cell culture dish holder	OX.9703 IR cut-off filter	OX.9714 Blue 550 nm interference filter 45 mm diameter for Oxion Inverso	OX.9787 Green 550 nm interference filter 45 mm diameter for Oxion Inverso
OX.9745 Filter block with filter set for excitation in Blue spectrum EX465-495 DM505LP EM515-555	OX.9746 Filter block with filter set for excitation in Green spectrum EX540-580 DM600 EM605-665	OX.9747 Filter block with filter set for excitation in UV spectrum EX361-389 DM415 EM435-485	OX.9748 Filter block with filter set for excitation in Violet spectrum EX415-445 DM455 EM465-495
OX.9749 Empty filter block for filters and dichroic mirror for Oxion Inverso inverted microscope	OX.9753 Filter block rail, 3-positions for Oxion Inverso inverted microscope for fluorescence with 2 empty blocks	OX.9754 Filter block rail, 4-positions for Oxion Inverso inverted microscope for fluorescence with 3 empty blocks	OX.9833 Photo port adapter with 0.33x lens for Oxion (revision 2) microscopes and 1/3 inch camera with C-mount
OX.9850 Photo port adapter with 0.5x lens for Oxion (revision 2) microscopes and 1/2 inch camera with C-mount	OX.9985 5 W NeoLED replacement unit	AE.3252.ND01 Neutral density filter, diameter 32 mm, ND 0.10 @ 80% transmittance, mounted in ring	AE.3252.ND02 Neutral density filter, diameter 32 mm, ND 0.20 @ 63% transmittance, mounted in ring
AE.3252.ND03 Neutral density filter, diameter 32 mm, ND 0.30 @ 50% transmittance, mounted in ring	AE.3252.ND04 Neutral density filter, diameter 32 mm, ND 0.40 @ 40% transmittance, mounted in ring	AE.3252.ND05 Neutral density filter, diameter 32 mm, ND 0.50 @ 32% transmittance, mounted in ring	AE.3252.ND06 Neutral density filter, diameter 32 mm, ND 0.60 @ 25% transmittance, mounted in ring
AE.3252.ND10 Neutral density filter, diameter 32 mm, ND 1.0 @ 10% transmittance, mounted in ring	AE.3252.ND13 Neutral density filter, diameter 32 mm, ND 1.3 @ 5% transmittance, mounted in ring	AE.5130 Universal SLR adapter with built-in 2x lens for standard 23.2 mm tube. Needs T2 adapter	AE.5025 T2 ring for Nikon D SLR digital camera
AE.5040 T2 ring for Canon EOS SLR digital camera	AE.3199 Glass fuses 3.15A 250V, 10pcs	AE.8000 Micromanipulator for intracytoplasmic sperm injection (ICSI) suitable with the Oxion Inverso inverted microscope. (Non in vitro medical device)	AE.8010 Heating stage with transparent glass plate for AE.8000 Micromanipulator, including digital temperature controller
PB.5155 Microscope slides 76 x 26 mm, ground edges, 50 pieces per pack	PB.5165 Cover glasses 18 x 18 mm, 0.13-0.17 mm, 100 pieces	PB.5157-W Microscope slides 76 x 26 mm, ground edges. White frosted side, 50 pieces per pack	PB.5157-B Microscope slides 76 x 26 mm, ground edges. Blue frosted side, 50 pieces per pack
PB.5160 Microscope slides 76 x 26 mm with cavity, grinded edges. 10 pieces per pack	PB.5168 Cover glasses 22 x 22 mm, 0.13-0.17 mm, 100 pieces	PB.5245 Lens cleaning paper, 100 sheets per pack	PB.5255 Immersion oil, 25 ml. Refraction index n = 1.515
PB.5255.0100 Immersion oil, 100 ml. Refraction index n = 1.515	PB.5274 Iso propyl alcohol 99%, 200 ml	PB.5275 Cleaning kit: lens fluid, lint free lens tissue/paper, brush, air blower, cotton swabs

Oxion Inverso (fluorescence)

The inverted Oxion Inverso biological microscopes of Euromex are available for bright field, phase contrast and fluorescence applications. Specific objectives compensate the 1.2 mm thick bottoms of the petri dishes or culture dishes

The Oxion Inverso inverted microscopes (Life Science) are useful for observation of living organisms and tissues cultures in cell culture dishes and flasks

Highlights

• Life Science applications
  
• Observation of tissue cultures, living cells
  
• Brightfield, phase contrast and fluorescence
  
• Long working distance IOS plan achromatic objectives
  
• Revolving quintuple nosepiece
  
• 5 W LED transmitted illumination
  
• 100 W mercury vapor lamp for fluorescence

Specifications

EYEPIECE(S)
EYEPIECESPair of DIN HWF plan 10x/22 mm eyepieces

HEAD

• Trinocular 45° inclined tubes with extended low and high positions for eyepieces
  
• One diopter adjustment on left eyepiece
  
• Interpupillary distance of 54 to 75 mm
  
• Trinocular head with 0-100 % positions

NOSEPIECE
Revolving quintuple nosepiece on ball-bearings

OBJECTIVES FOR BRIGHTFIELD 
(models OX.2003-PL and OX.2053-PL)

• Infinity color corrected long working distance plan 10x/0.25, 20x/0.40, 40x/0.60 IOS objectives
  
• An optional 60x/0.70 IOS objective is also available

OBJECTIVES FOR PHASE CONTRAST
(models OX.2003-PLPH and OX.2053-PLPH)

Infinity color corrected long working distance plan phase 10x/0.25, 20x/0.40, 40x/0.60 IOS objectives 
(Photo: OX.9190 Slider with 4x/10x and 20x/40x phase contrast annuli)

OBJECTIVES FOR BRIGHTFIELD AND FLUORESCENCE MICROSCOPY
(models OX.2253-PLPHF and OX.2453-PLPHF)

• Infinity color corrected long working distance plan Fluarex 10x/0.30, 20x/0.45, 40x/0.65 IOS objectives
  
• An optional plan Fluarex 60x/0.75 IOS objective is also available

OBJECTIVES FOR PHASE CONTRAST AND FLUORESCENCE MICROSCOPY
(models OX.2253-PLPHF and OX.2453-PLPHF)

Infinity color corrected long working distance plan fluarex 10x/0.30 and plan phase fluarex 20x/0.45 and S40x/0.65 IOS objectives. 

All objectives corrected for 1.2 mm thickness. 
All optics are anti-fungus treated and anti-reflection coated for maximum light throughput 

STAGE
Stage 250 x 230 mm equipped with a coaxial mechanical 120 x 78 mm X-Y stage, delivered with:

• metallic insert for Ø 35 mm cell culture dish dishes
  
• 76 x 26 mm slide holder
  
• lass insert with hole

optional universal cell culture dish holder
(Photo: OX.9599 Universal cell structure dish holder)

CONDENSER

• N.A. 0.30 condenser with iris diaphragm. Working distance 72 mm
  
• The models for the phase contrast OX.2003-PLPH, OX.2053- PLPH, OX.2253-PLPHF and OX.2453-PLPHF are delivered with a slider with 4x/10x and 20x/40x pre-centered phase rings

FOCUSING ADJUSTMENT
Coaxial 25 mm coarse and fine adjustments, 2 μm precision and with friction adjustment

ILLUMINATION

• Transmitted 5 W LED Intensity adjustable with internal 100-240 V power supply. Fuse holder 3.15 A / 250 V. 
  
• The fluorescence models are equipped with a reflected 100 W HBO mercury vapor light source with external 100-240 V power supply, fuse holder 3.15 A / 250 V, UV protection shield

LED ILLUMINATION FOR FLUORESCENCE
Two LED sources for fluorescence are available for 4 or 9 excitation wavelengths.
See our product LE.6400-OX

FILTERS FOR PHASE CONTRAST MODELS
Interference filter 550 nm green and blue filter, 45 mm diameter

STANDARD FLUORESCENCE FILTERS
(for fluorescence models)

The fluorescence models OX.2253-PLF and OX.2253-PLPHF are standard supplied with two filter sets for excitation:

• in the blue spectrum, OX.9745, EX465-495 (BP) DM505 (LP) EM515-555 (BP)
  
• in the green spectrum, OX.9746, EX540-580 (BP) DM600 (LP) EM605-665 (BP)

The fluorescence models OX.2453-PLF and OX.2453-PLPHF equipped with a 4 positions filter rail equipped with three standard filter sets for excitation:

• in the blue spectrum, OX.9745, EX465-495 (BP) DM505 (LP) EM515-555 (BP)
  
• in the green spectrum, OX.9746, EX540-580 (BP) DM600(LP) EM605-665 (BP)
  
• in the UV spectrum, OX.9747, EX361-389 (BP) D415 (LP) EM435-485 (BP)

STANDARD GENERAL PURPOSE FLUORESCENCE FILTERS SETS

OX.9745 Blue excitation 465-495 nm 
FITC EX465-495 (BP) DM505 (LP) EM515-555 (BP)

Also suitable for: Acridine Orange + DNA, Alexa Fluor 488™, Atto 488, Azami Green, BODIPY FL/pH7.2, Calcein, Calcium Green™-1, Cy2™, DiO, DyLight 488, EGFP, Emerald GFP, FAM, FITC, Fluo-4, GFP LysoTracker Green/pH 5.2, MitoTracker Green FM/MeOH, mWasabi, Oregon Green™ 488, SYBR® Green I, SYTO 9/DNA, ZsGreen

OX.9746 Green excitation 540-580 nm
Texas Red / mCherry / Alexa Fluor 594 EX540-580 (BP) DM600 (LP) EM605-665 (BP)

Also suitable for: Alexa Fluor 568™, Alexa Fluor 594™, CAL Fluor® Red 610, Cy3.5™, DyLight 594, FusionRed, Killer Red, mCherry, MitoTracker Red/MeOH, mKate2, mRFP1, ReAsH-CCPGCC, Rhodamine Red™-X, ROX, Sulforhodamine 101, Texas Red®, Texas Red®-X, X-rhod-1/Ca2+

OX.9747 UV excitation 361-381 nm
DAPI / Hoechst / Alexa Fluor 350 EX361-389 (BP) D415 (LP) EM435-485 (BP)

Also suitable for: Alexa Fluor 350™, Biosearch Blue, Coumarin, DAPI, DyLight 350, Hoechst 33258, LysoTracker Blue/MeOH

OX.9748 Violet excitation 361-389 nm
UV / DAPI Longpass, Calcofluor White EX415-445 (BP) DM455 (LP) EM465-495 (BP)

PACKAGE CONTENT

• Supplied with power cord, dust cover and user manual
  
• Phase contrast models are supplied with green filter and alignment telescope
  
• Microscope supplied in a transport case

ISCI (INTRACYTOPLASMIC SPERM INJECTION) SET FOR OXION INVERSO

(Photo: OX.2003-PLPH-ISCI Oxion Inverso with ICSI equipment for Intracytoplasmic Sperm Injection)

TECHNICAL SPECIFICATIONS

• Perfect for inexperienced users in need of training practice as well as for professionals
  
• Complete manual system
  
• Large movement range
  
• Easy micropipette position at each time ‘Manual Home Position’
  
• Adjustable micropipette holder angle between 20° and 40°
  
• Micropipette holder fixing knob reduces vibration and easy handling of the sperm
  
• Easy installation and service
  
• 3D Hydraulic Manipulation Joysticks for smooth movement
  
• Pneumatic Air Injector for ICSI and HOLDING
  
• No service required for injector
  
• Identical sensitive injectors on both sides and interchangeable
  
• Top quality for affordable price
  
• Movement Range: X/Y/Z: 20 mm each, 1 mm for one complete turn of the handle
  
• Optional heating stage with controller (Photo: ISCI set (detail)

ISCI SET
Set includes two 3D hydraulic joysticks, two pipette setup mechanisms, one connection bar with moving handle, two air injectors with tubing and pen type micromanipulation, pipette holder, two metal plates for injectors, one set of Allen wrenches, one ruler for mechanic home position adjustment.  Unit is packed in a solid carry case

HEATING STAGE

• Heating glass stage for Euromex microscope stage with 110 mm circular hole
  
• The glass plate fits the circular opening of the X-Y stage and enables a flat glass object-plate with constant temperature
  
• Aluminium frame for superior resistance and flatness
  
• Glass thickness 0.5 mm. Plate diameter 110 mm

CONTROLLER

• 2-Channel Temperature Controller with touchscreen interface
  
• One controller can control up to two devices
  
• External temperature sensor (included) and self calibration routines allows easy calibration of the heating devices
  
• On-board data logging allows to record temperature as a function of time and to log calibration events and alarms
  
• The password protected controller is equipped with routines to re-calibrate the sensors against certified thermometers

FEATURES

• Temperature range: from ambient to 60°C
  
• Temperature accuracy on specimen: ± 0.3°C
  
• 4.3’’ touch screen interface
  
• External Temperature sensor
  
• On-board Data logging to USB drive
  
• Alarm buzzer and External Alarm Connector
  
• One controller can control up to two devices
  
• Self calibration routines enable high temperature accuracy

Downloads

  Oxion_Inverso_LS_product_datasheet_English

  Fluorescence filter sets_datasheet_English 

https://www.euromex.com/en/products/applications/textile/textile-industry-applications/oxion-inverso-fluorescence/