Clostridium difficile (ribotype 027)

Cat no. YSL-qP-EC-C.diff027-100

Human, qPCR, SinglePlex

A complete qPCR kit for the detection of Clostridium difficile (ribotype 027)

Clostridium difficile (ribotype 027) is a highly virulent bacterium that causes severe diarrhea often associated with antibiotic use. Commonly known as C. difficile, this pathogen is a significant concern in healthcare settings, leading to prolonged hospital stays, increased treatment costs, and even mortality if not detected early. The bacterium can be found in both human and animal sectors, making it crucial to monitor and control its spread to prevent outbreaks.

YouSeq's qPCR kit to detect Clostridium difficile (ribotype 027) allows for rapid and accurate identification of this pathogen, enabling healthcare professionals to promptly initiate appropriate treatment and infection control measures. Early detection is essential in preventing the spread of C. difficile infections, ultimately reducing the burden on healthcare systems and improving patient outcomes.

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Contents

• Target Specific primer/probe
  
• Endogenous control primer/probe
  
• qPCR OneStep MasterMix
  
• Positive control template
  
• DNase/RNase free water
  
• Template resuspension buffer
  
• ROX passive reference

Technical specification
Target Gene(s):tcdE gene
Efficiency: 90-110%
Sensitivity: <100 copy detection
Run Time: Approx 1hr30 (dependent on instrumentation)
Target Pathogen: FAM labelled
Endogenous Control: VIC/HEX labelled

Pseudonyms
C. difficile; CDI; Clostridium difficile infection; Ribotype 027; C.diff; Clostridioides difficile; CDF; Clostridium difficile-associated diarrhea; Clostridium difficile colitis; Toxic megacolon