3,3′,5,5′-Tetramethylbenzidine dihydrochloride;
TMB·HCl;
TMB dihydrochloride
MOLECULAR BIOLOGY GRADE
C16H20N2 ·HCl · xH2O
MW: 313.27 g/mol (anhydrous)
TMB is an excellent colorimetric substrate for detection of horseradish peroxidase labeled probes and is used with peroxidase and peroxidase-coupled systems, particularly in ELISA techniques. It produces a soluble end product, which is blue in color and can be read spectrophotometrically. TMB has also been used in the quantitative determination of hemoglobin and in cytochemical staining for peroxidase. TMB is a hepatitis B antigen.
Storage/Handling: Store dry at -15°C, and protect from light and humidity.
