Small RNA is a group of RNA molecules that include microRNAs (miRNAs), small interfering RNAs (siRNAs), and piwi-interacting RNAs (piRNAs). Among these, miRNAs, around 18-25 nucleotides long, are particularly noteworthy for their pivotal regulatory roles in various cellular processes. With tissue-specific and stage-specific expression patterns, miRNAs exhibit high conservation across different species.

Platform: Illumina NovaSeq

  Features

- Size selection library preparation.
- Bioinformatic analysis centered around miRNA prediction and targets.

Service Advantages 

- Extensive Expertise: We have processed over 300 samples, spanning diverse sample types. We bring the wealth of expertise to every project.
- Rigorous Quality Control: We implement core control points across all stages, from sample preparation to library preparation, sequencing and bioinformatics. Our meticulous monitoring ensures the delivery of consistently high-quality results.
- Comprehensive bioinformatics analysis: It enables the identification of both known and novel miRNAs, identification of miRNAs targets, and corresponding functional annotation and enrichment with multiple databases (KEGG, GO).
- Post-Sales Support: We understand how important is to be present, that’s why our commitment extends beyond project completion with a 3-month after-sale service period. During this time, we offer project follow-up, troubleshooting assistance, and Q&A sessions to address any queries related to the results.

  Sample Requirements and Delivery

  Sample Requirements:

Nucleotides:

- Plants:
   - Root, Stem or Petal: 450 mg
   - Leaf or Seed: 300 mg
   - Fruit: 1.2 g

- Animal:
   - HEart or Intestine: 450 mg
   - Viscera or Brain: 240 mg
   - Muscle: 600 mg
   - Bones, Hair or Skin: 1.5g

- Arthropods:
   - Insects: 9g
   - Crustacea: 450 mg

- Whole blood: 2 tube

- Cells: 10⁶ cells

- Serum and Plasma: 6 mL

Recommended Sample Delivery

Container: 2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3.

Shipment:

1. Dry-ice: Samples need to be packed in bags and buried in dry-ice.

2. RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature

  Service Work Flow

Experiment design	Sample delivery	RNA extraction	Library construction	Sequencing	Data analysis	After-sale services

- Raw data quality control
- Small RNA classification
- miRNA expression and differential expression analysis
- miRNA target gene and differentially expressed miRNA target gene annotation
- Target gene annotation and differentially expressed miRNA gene function enrichment

Identification of miRNA: structure and depth

Differential expression of miRNA – hiearchical clustering

Functional annotation of target of differentially expressed miRNAs

Explore the research advancements facilitated by BMKGene’ sRNA sequencing services through a curated collection of publications.

Chen, H. et al. (2023) ‘Viral infections inhibit saponin biosynthesis and photosynthesis in Panax notoginseng’, Plant Physiology and Biochemistry, 203, p. 108038. doi: 10.1016/J.PLAPHY.2023.108038.

Li, H. et al. (2023) ‘ The plant FYVE domain‐containing protein FREE1 associates with microprocessor components to repress miRNA biogenesis ’, EMBO reports, 24(1). doi: 10.15252/EMBR.202255037/SUPPL_FILE/EMBR202255037-SUP-0004-SDATAFIG4.TIF.

Yu, J. et al. (2023) ‘The MicroRNA Ame-Bantam-3p Controls Larval Pupal Development by Targeting the Multiple Epidermal Growth Factor-like Domains 8 Gene (megf8) in the Honeybee, Apis mellifera’, International Journal of Molecular Sciences, 24(6), p. 5726. doi: 10.3390/IJMS24065726/S1.

Zhang, M. et al. (2018) ‘Integrated Analysis of MiRNA and Genes Associated with Meat Quality Reveals that Gga-MiR-140-5p Affects Intramuscular Fat Deposition in Chickens’, Cellular Physiology and Biochemistry, 46(6), pp. 2421–2433. doi: 10.1159/000489649.