Dictyostelium Discoideum is capable to grow axenically in A Medium without bacteria as food. Peptone and Yeast Extract are the main components of this medium. Peptone provides high molecular weight peptides and proteins as a nitrogen source. Yeast Extract is a source of vitamins, co-factors and carbohydrates. An additional Phosphate buffer is present to inhibit acidification of the medium during cell growth.
Suspend 13.7 gram powdered medium in 1 litre distilled water
Store dry at room temperature
https://www.formedium.com/product/a-medium/?attribute_pa_size=250g
