OneScriptPlus cDNA Synthesis Kit,100 x 20 ul reactions

คุณสมบัติสินค้า:

SKU : G236

แบรนด์ : ABM Good (Canada)

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Description
OneScript® Plus cDNA Synthesis Kit contains a Moloney-Murine Leukemia Virus Reverse Transcriptase with genetic modifications to abolish RNase H activity to achieve thermal stability. This special mutant enzyme offers higher cDNA yields, longer cDNA up to 12 kb, and is able to perform under high temperatures (50°C - 55°C), facilitating the elimination of secondary structures associated with GC-rich RNA templates. OneScript® Plus is formulated with abm’s RNaseOFF Ribonuclease Inhibitor offering improved resistance to oxidation compared to the high oxidation-sensitive human RNase inhibitors. RNaseOFF is stable even under very low concentrations of DTT (< 1 mM), making it the best choice for ultimate RNA protection.

Key Features

  • Maximal flexibility in priming – oligo(dT), random primers or gene-specific primers
  • Robust cDNA synthesis from any RNA template
  • High reproducibility and excellent yield 
  • Employs abm's OneScript® Plus Reverse Transcriptase.

Product ComponentQuantity
OneScript® Plus Reverse Transcriptase100 rxn (100 µl)
5X RT Buffer400 µl
Oligo(dT) (10 μM)100 µl
Random Primers (10 μM)100 µl
dNTP (10 mM)100 µl
Nuclease-Free H2O2 x 1.0 ml
 

 SKU G236

Applications

  • Synthesizing cDNA from ssRNA
  • DNA primer extension
  • Sequencing dsDNA
  • Constructing cDNA library
  • Producing template for use in RT-PCR
  • Labelling 3'-end of duplex DNA via end-filling reactions
  • Generating probes for hybridization

Storage Condition : Store at -20°C.
Unit quantity : 100 rxn

Material Citation : If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G236

https://www.abmgood.com/onescript-plus-cdna-synthesis-kit.html#G236

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