OneScript Plus cDNA Synthesis Kit, 25 x 20 ul reactions

Cat. No. : G236Print
Name : OneScript® Plus cDNA Synthesis Kit
Unit : 100 rxn
Category : Reverse Transcriptase & RT-PCR
Description : (Equivalent to Cat. No. G233, G234, G235, and G491.)
OneScript® Plus cDNA Synthesis Kit contains a Moloney-Murine Leukemia Virus Reverse Transcriptase with genetic modifications to abolish RNase H activity to achieve thermal stability. This special mutant enzyme offers higher cDNA yields, longer cDNA up to 12 kb, and is able to perform under high temperatures (50°C - 55°C), facilitating the elimination of secondary structures associated with GC-rich RNA templates. OneScript® Plus is formulated with abm’s RNaseOFF Ribonuclease Inhibitor offering improved resistance to oxidation compared to the high oxidation-sensitive human RNase inhibitors. RNaseOFF is stable even under very low concentrations of DTT (< 1 mM), making it the best choice for ultimate RNA protection.

 Key Features

• Maximal flexibility in priming – oligo(dT), random primers or gene-specific primers
  
• Robust cDNA synthesis from any RNA template
  
• High reproducibility and excellent yield 
  
• Employs abm's OneScript® Plus Reverse Transcriptase (Cat. No. G237)
  
• Earn 2X the rewards points

Application

• Synthesizing cDNA from ssRNA
  
• DNA primer extension
  
• Sequencing dsDNA
  
• Constructing cDNA library
  
• Producing template for use in RT-PCR
• Labelling 3'-end of duplex DNA via end-filling reactions
  
• Generating probes for hybridization

Storage Condition : Store at -20°C.
Material Citation : If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G236

https://www.abmgood.com/onescript-plus-cdna-synthesis-kit.html#G236