Presto™ 96 Well PCR Cleanup Kit

DNA Extraction/PCR CleanupPresto™ 96 Well PCR Cleanup Kits were designed for DNA fragments purification from PCR or other enzymatic reactions using an efficient 96 well binding plate system. This PCR Purification Kit uses chaotropic salt to denature enzymes, allowing DNA fragments to be bound by the glass fiber matrix in each well of the 96 well binding plate. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer, TE or water. Salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation and the purified DNA is ready for use in subsequent reactions. The Presto™ 96 Well PCR Cleanup Kit is a cost effective, high performance alternative for efficient DNA fragments PCR purification.

DESCRIPTION
Specifications (Cat. # 96DFH04, 96DFH10)

• Purify up to 300 mg of agarose gel slices per well in 45 minutes!
  
• Purify up to 100 µl of PCR products per well in 20 minutes!
  
• High Recovery: up to 95%
  
• Format: Presto™ PCR Cleanup 96 Well Binding Plates
  
• Broad Fragment Size Range: 70 bp–20 kb
  
• Centrifuge or vacuum manifold protocol
  
• Elution Volume: 60 µl from 80 µl elution buffer volume, 40 µl from 60 µl elution buffer volume
  
• Storage: dry at room temperature (15-25ºC)

APPLICATIONS
PCR, Fluorescent or Radioactive Sequencing, Restriction Enzyme Digestion, DNA Labeling and Ligation

COMPONENTS

• Binding Buffer
  
• Wash Buffer
  
• Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
  
• Presto™ PCR Cleanup 96 Well Binding Plates
  
• 0.35 ml Collection Plates
  
• Adhesive Film

QUALITY CONTROL
The quality of the Presto™ 96 Well PCR Cleanup Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. DNA fragments of various sizes are purified from PCR products and analyzed by electrophoresis.

 DOCUMENTS

Presto™ 96 Well PCR Cleanup Kit

PrestoTM 96 Well PCR Cleanup Kit Quick Protocol

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