DNA Extraction/Genomic DNA PurificationThe Genomic DNA Mini Kit (Blood/Cultured Cell) is a genomic DNA extraction kit optimized for genomic, mitochondrial and virus DNA purification from whole blood, buffy coat and cultured cell samples. This Genomic DNA Extraction Kit uses RBC Lysis Buffer and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the genomic DNA spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. Phenol/chloroform extraction or alcohol precipitation is not required and the purified genomic DNA (approximately 20-30 kb) is suitable for use in PCR or various other enzymatic reactions. For RNA purification from blood and cultured cell samples, the Total RNA Mini Kit (Blood/CC) is also available.
Specifications (Cat. # GB100, GB300)
PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR
The quality of the Genomic DNA Mini Kit (Blood/Cultured Cell) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Genomic DNA from 300 μl of whole human blood is extracted using the Genomic DNA Mini Kit (Blood/Cultured Cell). The purified DNA (4-10 μg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.