Presto™ Stool DNA Extraction Kit

DNA Extraction/Genomic DNA PurificationThe Presto™ Stool gDNA Extraction Kit is designed for rapid isolation of genomic DNA from microorganisms, such as bacteria and fungi in stool samples. The stool sample is homogenized and disrupted using a lysis buffer and ceramic beads. Insoluble particles, proteins and PCR inhibitors such as humic acid are then precipitated with a proprietary inhibitor removal buffer. Residual inhibitors are further removed by passing through a specialized PCR inhibitor removal column. Genomic DNA in the sample is then bound by the GD Column followed by wash and elution. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 50 minutes. The purified genomic DNA is ready for use in PCR, restriction enzyme digestion, and sequencing reactions.

DESCRIPTION
Specifications (Cat. # STLD050, STLD100)

• Purify genomic DNA from stool samples within 50 minutes!
  
• Sample: 180-200 mg of fresh or frozen stool samples
  
• Binding Capacity: 50 µg
  
• High Quality DNA: A260/A280 = 1.8-2.0
  
• Format: beadbeating tubes, inhibitor removal columns and spin columns
  
• Elution Volume: 30-100 µl
  
• Storage: dry at room temperature (15-25°C)

APPLICATIONS
PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR

COMPONENTS

• ST1 Buffer
  
• ST2 Buffer
  
• ST3 Buffer
  
• Wash Buffer
  
• Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
  
• Inhibitor Removal Columns
  
• GD Columns
  
• Beadbeating Tubes Type C
  
• 2 ml Centrifuge Tubes
  
• 2 ml Collection Tubes

QUALITY CONTROL
The quality of the Presto™ Stool DNA Extraction Kit is tested on a lot-to-lot basis by isolating genomic DNA from 200 mg stool samples. Following the purification process, the purified genomic DNA (A260/A280 ratio between 1.8-2.0) is analyzed by electrophoresis.

DOCUMENTS

Presto™ Stool DNA Extraction Kit

PrestoTM Stool DNA Extraction Kit Quick Protocol

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