gSYNC™ 96 Well DNA Extraction Kit

96 Well DNA/RNA Extraction/96 Well DNA ExtractionThe 96 Well gSYNC™ DNA Extraction Kit is optimized for high-throughput genomic DNA extraction from tissue and cells. This DNA extraction kit uses Proteinase K and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the 96 Well Binding Plate. Contaminants are removed using a Wash Buffer and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed within 60 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA (approximately 20-30 kb) is suitable for use in PCR or other enzymatic reactions.

DESCRIPTION
Specifications (Cat. # 96GS004, 96GS010)

• Purify 96 wells of genomic DNA within 1 hour!
  
• Variety of Samples: tissue, rodent tails, ear punches and cultured cells
  
• High Yield: 10-20 µg from 20 mg of tissue
  
• High Quality DNA: A260/A280 = 1.8-2.0
  
• Spin Columns: glass fiber membrane optimized for genomic DNA extraction
  
• Elution Volume: 100-400 µl
  
• Storage: dry at room temperature (15-25°C)

APPLICATIONS
PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR

COMPONENTS

• GST Buffer
  
• GSB Buffer
  
• W1 Buffer
  
• Wash Buffer
  
• Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
  
• Proteinase K
  
• RNase A
  
• 96 Well gDNA Binding Plates
  
• Microtubes (Racked)
  
• Microtubes (8-strip)
  
• Caps for Microtubes (8-strip)
  
• 96 Deep Well Plates

QUALITY CONTROL
The quality of the 96 Well gSYNC™ DNA Extraction Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Genomic DNA from 200 μl of whole human blood is extracted using the gSYNC™ DNA Extraction Kit. The purified DNA (5 μg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.

DOCUMENTS

gSYNC™ 96 Well DNA Extraction Kit

gSYNCTM 96 Well DNA Extraction Kit Quick Protocol

https://www.geneaid.com/product.php?act=view&id=194