ZEROprep™ DNA Reagent

Reagent DNA Extraction/Reagent DNA ExtractionZEROprep™ DNA PCR Reagent is designed for efficient release of DNA for direct use in PCR reactions without purification. A wide variety of samples are effectivly homogenized in the reagent without any pre-treatment or subsequent bind, wash or elution steps. Simply place the sample in the reagent, follow the 2 step protocol and transfer the lysate to a PCR mix.

DESCRIPTION
Specifications (Cat. # ZDR050, ZDR100)

• Use DNA directly in PCR reactions
  
• DNA purification is not required
  
• 15 minute, 2 step protocol
  
• Process a wide variety of sample types (tissue, blood, plant, bacteria, yeast/fungus, virus etc.)
  
• Storage: dry at room temperature (15-25ºC)

APPLICATIONS
PCR, Multiplex PCR, Real-time PCR

COMPONENTS

• ZEROprep™ DNA PCR Reagent

QUALITY CONTROL
ZEROprep™ DNA PCR Reagent is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. DNA from a 1 mg tissue sample is lysed in ZEROprep™ DNA PCR Reagent. A 5 µl aliquot of lysate is added directly into a 50 µl PCR mix.

ZEROprep™ DNA PCR Reagent PCR Data

Figure 1 .Human blood samples were lysed in ZEROprep™ DNA PCR Reagent for 15 minutes at room temperature. Plant and bacteria samples were lysed for 15 minutes at 80ºC. 2 μl aliquots of lysate were added to 20 μl PCR mixtures. Lane 1-2: partial human ACTB gene (250 bp) was amplified from 10 μl of human blood. Lane 3-4: partial Actin-2 gene (750 bp) was amplified from 20 mg of Arabidopsis thaliana leaves. Lane 5: partial 16S ribosomal gene (200 bp) was amplified from 2 μl of cultured Escherichia coli broth. Lane 6: partial 16S ribosomal gene (200 bp) was amplified from 2 μl of cultured Bacillus subtilis broth.

DOCUMENTS

ZEROprep™ DNA Reagent

https://www.geneaid.com/product.php?act=view&id=254