Sulfonamides ELISA Test Kit, Veterinary Drugs, 0.5 ppb

Sulfonamides ELISA Test Kit for Poultry Tissues, Aquatic Tissue and Serum

The Sulfonamides ELISA Kit is competitive ELISA for the qualitative or quantitative analysis of sulfonamides in samples of poultry tissues, aquatic tissue, serum, etc.

Description

Summary

Sulfonamides are group of synthetic antibiotic agents that have been widely used for therapeutic and prophylactic purposes in both animals and human.

They are used as feed additives as well, mainly for fattening of calves and pigs. SAs have seen an emergence in the aquaculture industry due to their

effectiveness against gram-negative and gram-positive organisms.

The detection method is sensitive, fast, simple, and specific, only need 45mins for operating and a small amount of sampling.

Use Principles

The Sulfonamides ELISA kit is an indirect competitive enzyme-labeled immunoassay. The antigen is precoated on the wells. The precoated antigen

compete the antibody (antibody solution) with sulfonamides in the sample, anti-sulfonamides antibody binds to the HRP enzyme conjugate. Then

pipette the substrate solution to the wells to convert the color. The color of unknown samples is compared to the color of the standards and the

sulfonamides concentration of the samples is derived.

SENSITIVITY (DETECTION LIMIT)

—- Sensitivity: 0.5ppb

Limit of detection

Tissue sample ……………………………………………………………………………….. 4ppb

Serum sample……………………………………………………………………………….. 5ppb

Cross-reactivity

Sulfadiazine …………………………………………………………………………………..100%

Sulfadoxine ……………………………………………………………………………………181%

Sulfamonomethoxine……………………………………………………………………..150%

Phthalylsulfathiazole (PST)……………………………………………………………….90%

Sulfamethoxydiazine………………………………………………………………………..85%

Sulfadimethoxypyrimidine ……………………………………………………………….67%

Benzene Sulfapyridine ……………………………………………………………………..62%

Sulfapyridine……………………………………………………………………………………55%

Sulfamerazine………………………………………………………………………………….52%

Sulfamethizole…………………………………………………………………………………49%

Sulfachlorpyridazine ……………………………………………………………………..29.6%

Sulfamethazine………………………………………………………………………………..13%

Percent Recovery, %

Tissue sample ………………………………………………………………………….. 80±10%

Serum sample………………………………………………………………………….. 90±15%

Precision (CV %):

Intra-lab assay: CV%≤10%

https://mzfoodtest.com/product/sulfonamides-elisa-test-kit-for-poultry-tissues-aquatic-tissue-and-serum/