Brucella broth

Brucella Broth [MB-B2134]

Medium used for the cultivation and enumeration of Brucella species and other fastidious microorganisms.

*  Equally use with MFDS (MB-B2134K).
* This product is identical to the food industry standards . 

PROCEDURE

Suspend 28.1 G of powder in 950 mL or 900 mL of distilled or deionized water. Heat to boiling until completely dissolved. Sterilize by autoclave at 121°C for 15 minutes. Cool to 45 - 50°C in water bath. Aseptically add 5 to 10% sterile Bovine Serum (MB-B1887, required heat inactivation at 56°C for 30 miniutes). If necessary, add 5 to 10% sterile Sheep Blood Defibrinated (MB-S1876) or Horse Blood Defibrinated (MB-H1883) instead of Bovine Serum (MB-B1887). Pour into tubes.

STORE

The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared medium at 2 - 8 ° C.