Azide Dextrose Broth

Azide Dextrose Broth [MB-A1003]

Selective medium used for the detection and enumeration of faecal Streptococci from waters and foodstuffs.

*  Equally use with NIER (MB-A1003N).
* This product is identical to the drinking water quality process standards .  

PROCEDURE

Suspend 34.7 G of powder in 1 L of distilled or deionized water or 69.4 G of powder in 1 L of distilled or deionized water for double strength medium. Heat to boiling until completely dissolved. Sterilize by autoclave at 121°C for 15 minutes. Cool to 45-50°C in water bath. Dispense in final containers. If necessary dispense the medium before autoclave. In the case of three times strength medium, dissolve 104.1 G of dehydrated medium in 1 L of distilled or deionized water and proceed as the single strength medium.

STORE

The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared medium at 2-8°C.