SKU : MB-T1556
หมวดหมู่ : 12. Lab Equipment , MB Cell ,
แบรนด์ : MB cell
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Terrific Broth [MB-T1556]
Medium used for the cultivation recombinant strains of Escherichia coli.
PROCEDURE
Suspend 47.6 G of powder in 1 L of distilled or deionized water. Add 4.0 mL of Glycerol supplement (MB-G1821). Heat to boiling until completely dissolved. Sterilize by autoclave at 121°C for 15 minutes. Cool to 45-50°C in water bath. Mix well. Dispense in tubes.
STORE
The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared medium at 2 - 8°C.
