SKU : MB-S0713
หมวดหมู่ : 12. Lab Equipment , MB Cell ,
แบรนด์ : MB cell
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SOB (Super Optimal Broth) [MB-S0713]
Medium used for the cultivation and enumeration of recombinant strains of Escherichia coli.
* Equally use with SOC (Super Optimal broth with Catabolite repression) Broth (MB-S0816).
PROCEDURE
Suspend 28.09 G of powder in 1 L of distilled or deionized water. Heat to boiling until completely dissolved. Sterilize by autoclave at 121°C for 15 minutes. Cool to 45 - 50°C in water bath. If necessary, aseptically add 20 mL of filter sterilized 20% glucose solution. (SOC Broth). Mix well. Pour into tubes.
STORE
The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared medium at 2 - 8°C.
