SKU : MB-R1177
หมวดหมู่ : 12. Lab Equipment , MB Cell ,
แบรนด์ : MB cell
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Rogosa Broth [MB-R1177]
Selective basal medium for lactobacilli isolation and enumeration.
PROCEDURE
Suspend 58.72 G of powder in 1 L of distilled or deionized water. Add 1 mL of Tween 80 supplement (MB-T1861) and 1.32 mL of Glacial Acetic Acid supplement (MB-G0743). Heat until completely dissolved. Boil for 2-3 minutes. Cool to 45-50°C. Dispense in sterile final tubes. Do not sterilize in autoclave and do not overheat.
STORE
The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared medium at 2 - 8°C.
