Perfringens Agar Base

Perfringens Agar Base [MB-P2224]

Medium used for the isolation and cultivation of Clostridium perfringens.

* Equally use with Clostridium Perfringens Agar Base (MB-C1207), Perfringens (OPSP) Agar (MB-P2223) and SFP (Shahidi Feruson Perfringens) Agar (MB-S0614).

Perfringens (OPSP) Agar - PROCEDURE

Suspend 47.0 G of powder in 1 L of distilled or deionized water. Heat to boiling until completely dissolved. Sterilize by autoclave at 121°C for 15 minutes. Cool to 45 - 50°C in water bath. Aseptically 2 vials of Perfringens Selective supplement, OPSP (MB-C2549). Mix well. Pour into petri dishes.

Perfringens (SFP / TSC) Agar - PROCEDURE

Suspend 47.0 G of powder in 950 mL of distilled or deionized water. Heat to boiling until completely dissolved. Sterilize by autoclave at 121°C for 15 minutes. Cool to 45 - 50°C in water bath. Aseptically add 50 mL of Egg Yolk Emulsion (MB-E1864) and 2 vials of Perfringens Selective supplement, SFP (MB-P2550) or Perfringens Selective supplement, TSC (MB-P2551). Mix well. Pour into petri dishes.

STORE

The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared medium at 2 - 8°C.