2% Ogawa Medium
คุณสมบัติสินค้า:
SKU : MB-O0982
หมวดหมู่ : 12. Lab Equipment , MB Cell ,
แบรนด์ : MB cell
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2% Ogawa Medium [MB-O0982]
Medium used for the isolation and cultivation of Mycobacterium spp.
PROCEDURE
Suspend 2.6 G of powder in 100 mL of distilled or deionized water. Add 4 mL of Glycerol supplement (MB-G1821) and 4 mL of Malachite Green Solution 2% (MB-M0745). Heat to boiling until completely dissolved. Sterilize by autoclave at 121°C for 15 minutes. Cool to 45 - 50°C in water bath. Aseptically and 200 mL of Egg-Homogenate (MB-E1810). Mix well. Pour into tubes to 5 mL. Arrange tubes in a slanted position. Coagulate and inspissate at 85 - 90°C for 60 minutes.
STORE
The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared medium at 2 - 8°C.
