SKU : MB-K0960
หมวดหมู่ : 12. Lab Equipment , MB Cell ,
แบรนด์ : MB cell
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KCN Broth [MB-K0960]
Medium used for the identification and determination of Enterobacteriaceae on the basis of KCN tolerance.
PROCEDURE
Suspend 13.87 G powder in 1 L of distilled or deionized water. If necessary, adjust pH to 7.6 ± 0.2. Heat to boiling until completely dissolved. Sterilize by autoclave at 121°C for 15 minutes. Cool to 45 - 50°C in water bath. Aseptically add 2 vials of KCN supplement (MB-K3018). Pour into tubes. KCN is highly toxic, so always be careful when using KCN. Work must be performed in a hood.
STORE
The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared medium at 2 - 8°C.
