G418 Sulfate Solution (50mg/mL), 5×1 mL

Product Information

Product Description

G418 Sulfate, also known as Geneticin, G418, G 418 or G-418, is an aminoglycoside antibiotic with a structure similar to Gentamycin B1. Derived from Micromonospora rhodorangea and commonly used to screen for eukaryotic or prokaryotic polyclonal or monoclonal cells expressing the neo genes. G418 is used not only for the screening of stable cell lines, but also for the maintenance of stable cell lines.

In bacteria, yeast, protozoa, worms, higher plants and mammals, genomycin inhibits or kills cells by inhibiting protein synthesis. The mechanism of action is that genomycin binds to the 70S and 80S ribosomes, thereby blocking peptide chain extension and interfering with protein synthesis. Its resistance genes (mainly neo genes) are located in transposons Tn601 (903) or Tn5 (of bacterial origin) and can be expressed in eukaryotic cells. Introducing these resistance genes into cells by recombinant genetic techniques, the causing the cells to express the resistance product amino-glycoside 3'-phosphotransferase (APH(3)II), thereby acquiring resistance to G418 for the screening and maintenance of prokaryotic or eukaryotic cells carrying the resistance gene.

This product is a 50 mg/mL solution that has been filtered and de-bacterized and can be used directly in cell culture. Commonly used working concentration is 100-500 μg/mL.

Storage and Shipping Conditions

Ship with wet ice; Store at -20, valid for 12 months.

Product Contents

Note

1. Please pay attention to aseptic operation when using to avoid contamination.

2. Do not use G418 with other antibiotics/antifungals (e.g. penicillin/streptomycin), since they are competitive inhibitors of G418. Other antibiotics can also be cross reactivity.

3. Thaw at 4°C and mix well before use. Avoid repeated freezing and thawing.

4. For your safety and health, please wear safety glasses, gloves and protective clothing.

For Research Use Only!

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