SKU : G4620-500ML
หมวดหมู่ : 2. Cell & Molecular Biology , Cell Culture Medium / Sera , Servicebio ,
แบรนด์ : Servicebio
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Product Information
Product Name | Cat. No. | Spec. |
M199, GlutaPlus | G4620-500ML | 500 mL |
Product Description/Introduction
M199, known as Medium 199, was originally designed for the culture of chicken embryo fibroblasts and is now widely used for the culture of various animal cells, including non-mammalian cells. Compared to other basal medium, M199 contains a number of unique components, including adenine, adenosine, hypoxanthine, thymine and other vitamins. Different balanced salt solution can be used, for example Earles Balanced Salts Solution for CO2 environments and Hanks Balanced Salts Solution for non-CO2 environments. M199 is especially suitable for the culture of non-transformed cells and is also commonly used for virology, rat pancreatic epithelial cells and mouse lens tissue.
This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Add 5-10% serum or serum-free additives depending on the cell type.
This product is sterilized by 0.1μm filter membrane, pH 7.0-7.4, contains Earles Balanced Salts Solution, 1000 mg/L D-glucose, L-alanyl-L-glutamine.
Storage and Shipping Conditions
Ship at room temperature; Store away from light at 2-8, valid for 12 months.
Note
1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.
2. For best results, do not freeze and thaw repeatedly.
3. For your safety and health, please wear safety glasses, gloves and protective clothing.
For Research Use Only!
G4620 M199 Formula
Components | Concentration | Components | Concentration | |||
mg/L | mM | mg/L | mM | |||
Amino acids | Inorganic salts | |||||
Glycine | 50.0 | 0.6667 | Calcium Chloride (CaCl2)(anhyd.) | 200.0 | 1.802 | |
L-Alanine | 25.0 | 0.2809 | Ferric Nitrate (Fe(NO3)3·9H2O) | 0.7 | 0.001733 | |
L-Arginine hydrochloride | 70.0 | 0.3318 | Magnesium Sulfate (MgSO4) (anhyd.) | 97.7 | 0.8139 | |
L-Asparagine acid | 30.0 | 0.2256 | Potassium Chloride (KCl) | 400.0 | 5.333 | |
L-Cysteine hydrochloride·H2O | 0.10 | 0.0006 | Sodium Bicarbonate(NaHCO3) | 2200.0 | 26.190 | |
L-Cystine 2HCl | 33.9 | 0.1083 | Sodium chloride (NaCl) | 6800.0 | 117.24 | |
L-Glutamic acid | 75.0 | 0.5102 | Sodium dihydrogen phosphate(NaH2PO4)(anhyd.) | 121.7 | 1.014 | |
L-Alanyl-L-Glutamine | 148.8 | 0.6849 | Other components | |||
L-Histidine hydrochloride·H2O | 21.9 | 0.1042 | D-Glucose (Dextrose) | 1000.0 | 5.556 | |
L-Hydroxyproline | 10.0 | 0.0763 | Phenol Red | 18.83 | 0.053135 | |
L-Isoleucine | 40.0 | 0.3053 | Cholesterol | 0.20 | 0.00051680 | |
L-Leucine | 60.0 | 0.4580 | Glutathione (reduced) | 0.05 | 0.00016287 | |
L-Lysine hydrochloride | 70.0 | 0.3825 | Sodium Acetate | 50.0 | 0.60976 | |
L-Methionine | 15.0 | 0.1007 | Tween 80 | 20 | - | |
L-Phenylalanine | 25.0 | 0.1515 | Hypoxanthine Na | 0.40 | 0.0029412 | |
L-Proline | 40.0 | 0.3478 | 2-deoxy-D-ribose | 0.50 | 0.003731 | |
L-Serine | 25.0 | 0.2381 | Adenine sulfate | 5.772 | 0.024752 | |
L-Threonine | 30.0 | 0.2521 | Adenosine 5-phosphate | 0.211 | 0.0005764 | |
L-Tryptophan | 10.0 | 0.0490 | Adenosine 5-triphosphate | 0.911 | 0.0016529 | |
L-Tyrosine disodium salt dihydrate | 58.0 | 0.2222 | Guanine hydrochloride | 0.30 | 0.00159574 | |
L-Valine | 25.0 | 0.2137 | Ribose | 0.50 | 0.0033333 | |
Vitamins | Thymine | 0.30 | 0.0023810 | |||
Ascorbic Acid | 0.050 | 0.0002841 | Uracil | 0.30 | 0.002679 | |
Biotin | 0.010 | 0.0000410 | Xanthine-Na | 0.34 | 0.002237 | |
Choline chloride | 0.50 | 0.003571 | ||||
D-Calcium pantothenate | 0.010 | 0.0000210 | ||||
Folic Acid | 0.010 | 0.0000227 | ||||
Menadione (Vitamin K3) | 0.01606 | 0.00005814 | ||||
Niacinamide | 0.0250 | 0.0002049 | ||||
Nicotinic Acid (Niacin) | 0.0250 | 0.0002033 | ||||
Para-Aminobenzoic Acid | 0.0500 | 0.0003650 | ||||
Pyridoxal hydrochloride | 0.0250 | 0.0001225 | ||||
Pyridoxine hydrochloride | 0.0250 | 0.0001214 | ||||
Riboflavin | 0.0100 | 0.00002660 | ||||
Thiamine hydrochloride | 0.0100 | 0.00002967 | ||||
Vitamin A (acetate) | 0.100 | 0.00030488 | ||||
Vitamin D2 (Calciferol) | 0.100 | 0.00025189 | ||||
Alpha Tocopherol phos. Na salt | 0.010 | 0.0000180 | ||||
I-lnositol | 0.050 | 0.0002778 | ||||
