DMEM, no glucose, no L-Glutamine, no HEPES, no sodium pyruvate, 500 mL

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SKU : G4528-500ML

แบรนด์ : Servicebio

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Product Information

Product Name

Cat. No.

Spec.

DMEM, no glucose, no L-Glutamine, no HEPES, no Sodium Pyruvate

G4528-500ML

500 mL

 

Product Description/Introduction

Dulbecco's Modified Eagle Medium (DMEM), is a modified medium based on MEM, is now widely used in various cell cultures. The DMEM medium was originally designed as low-glucose containing D-Glucose 1000 mg/L, and later developed as high-glucose  containing D-Glucose 4500 mg/L. DMEM medium without D-glucose can be adjusted to suit the needs of the study.

This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Add 5-10% serum or serum-free additives depending on the cell type.

This product is sterilized by 0.1μm filter membrane, pH 7.,0-7.4, contains phenol red indicator, no D-glucose, L-glutamine, sodium pyruvate and HEPES buffer system.

Storage and Shipping Conditions

Ship at room temperature; Store away from light at 2-8, valid for 12 months.

Note

1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.

2. Avoid freezing the culture medium. Freezing may cause precipitation and precipitation of nutrients, which may not be completely dissolved after rewarming, affecting the cell culture effect. In addition, when the medium is stored at 2-8, do not store it completely close to the inner wall of the refrigerator to prevent the medium from freezing due to localized low temperature.

3. For your safety and health, please wear safety glasses, gloves, or protective clothing.

 

For Research Use Only! 

DMEM, no Glucose Formula

Components

Concentration


Components

Concentration

mg/L

mM

mg/L

mM

Amino acids




Inorganic salts



Glycine

30

0.4


Calcium Chloride (CaCl2) (anhyd.)

200

1.80

L-Arginine hydrochloride

84

0.4


Ferric nitrate nonahydrate ( Fe(NO3)39H2O)

0.1

0.0002

L-Cystine dihydrochloride

62.6

0.2


Magnesium Sulfate (MgSO4) (anhyd.)

97.67

0.80

L-Histidine hydrochloride-H2O

42

0.2


Potassium Chloride (KCl)

400

5.36

L-Isoleucine

105

0.80


Sodium Bicarbonate (NaHCO3)

3700

44.04

L-Leucine

105

0.80


Sodium Chloride (NaCl)

4750

81.9

L-Lysine hydrochloride

146

0.8


Sodium Phosphate monobasic (NaH2PO4) (anhyd.)

108.7

0.906

L-Methionine

30

0.2


Other components



L-Phenylalanine

66

0.4


D-Glucose (Dextrose)

-

-

L-Serine

42

0.4


Sodium pyruvate

-

-

L-Threonine

95

0.8


HEPES

-

-

L-Tryptophan

16

0.08


Phenol Red

14.95

0.0422

L-Tyrosine disodium salt dihydrate

103.79

0.4





L-Valine

94

0.8





L-Glutamine

-

-





L-Alanyl-L-Glutamine

  



Vitamins







Choline chloride

4.0

0.0286





D-Calcium pantothenate

4.0

0.0084





Folic Acid

4.0

0.0091





Niacinamide

4.0

0.0328





Pyridoxine hydrochloride

4.0

0.0195





Riboflavin

0.4

0.0011





Thiamine hydrochloride

4.0

0.0119





i-Inositol

7.2

0.0389





 

 

 

 

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