SKU : G4528-500ML
หมวดหมู่ : 2. Cell & Molecular Biology ,  Cell Culture Medium / Sera ,  Servicebio , 
แบรนด์ : Servicebio
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Product Information
Product Name | Cat. No. | Spec. |
DMEM, no glucose, no L-Glutamine, no HEPES, no Sodium Pyruvate | G4528-500ML | 500 mL |
Product Description/Introduction
Dulbecco's Modified Eagle Medium (DMEM), is a modified medium based on MEM, is now widely used in various cell cultures. The DMEM medium was originally designed as low-glucose containing D-Glucose 1000 mg/L, and later developed as high-glucose containing D-Glucose 4500 mg/L. DMEM medium without D-glucose can be adjusted to suit the needs of the study.
This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Add 5-10% serum or serum-free additives depending on the cell type.
This product is sterilized by 0.1μm filter membrane, pH 7.,0-7.4, contains phenol red indicator, no D-glucose, L-glutamine, sodium pyruvate and HEPES buffer system.
Storage and Shipping Conditions
Ship at room temperature; Store away from light at 2-8, valid for 12 months.
Note
1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.
2. Avoid freezing the culture medium. Freezing may cause precipitation and precipitation of nutrients, which may not be completely dissolved after rewarming, affecting the cell culture effect. In addition, when the medium is stored at 2-8, do not store it completely close to the inner wall of the refrigerator to prevent the medium from freezing due to localized low temperature.
3. For your safety and health, please wear safety glasses, gloves, or protective clothing.
For Research Use Only!
DMEM, no Glucose Formula
Components | Concentration | Components | Concentration | |||
mg/L | mM | mg/L | mM | |||
Amino acids | Inorganic salts | |||||
Glycine | 30 | 0.4 | Calcium Chloride (CaCl2) (anhyd.) | 200 | 1.80 | |
L-Arginine hydrochloride | 84 | 0.4 | Ferric nitrate nonahydrate ( Fe(NO3)39H2O) | 0.1 | 0.0002 | |
L-Cystine dihydrochloride | 62.6 | 0.2 | Magnesium Sulfate (MgSO4) (anhyd.) | 97.67 | 0.80 | |
L-Histidine hydrochloride-H2O | 42 | 0.2 | Potassium Chloride (KCl) | 400 | 5.36 | |
L-Isoleucine | 105 | 0.80 | Sodium Bicarbonate (NaHCO3) | 3700 | 44.04 | |
L-Leucine | 105 | 0.80 | Sodium Chloride (NaCl) | 4750 | 81.9 | |
L-Lysine hydrochloride | 146 | 0.8 | Sodium Phosphate monobasic (NaH2PO4) (anhyd.) | 108.7 | 0.906 | |
L-Methionine | 30 | 0.2 | Other components | |||
L-Phenylalanine | 66 | 0.4 | D-Glucose (Dextrose) | - | - | |
L-Serine | 42 | 0.4 | Sodium pyruvate | - | - | |
L-Threonine | 95 | 0.8 | HEPES | - | - | |
L-Tryptophan | 16 | 0.08 | Phenol Red | 14.95 | 0.0422 | |
L-Tyrosine disodium salt dihydrate | 103.79 | 0.4 | ||||
L-Valine | 94 | 0.8 | ||||
L-Glutamine | - | - | ||||
L-Alanyl-L-Glutamine | ||||||
Vitamins | ||||||
Choline chloride | 4.0 | 0.0286 | ||||
D-Calcium pantothenate | 4.0 | 0.0084 | ||||
Folic Acid | 4.0 | 0.0091 | ||||
Niacinamide | 4.0 | 0.0328 | ||||
Pyridoxine hydrochloride | 4.0 | 0.0195 | ||||
Riboflavin | 0.4 | 0.0011 | ||||
Thiamine hydrochloride | 4.0 | 0.0119 | ||||
i-Inositol | 7.2 | 0.0389 | ||||