Ham's F-12,GlutaPlus, 500 mL

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SKU : G4562-500ML

แบรนด์ : Servicebio

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Product Information

Product Name

Cat. No.

Spec.

Hams F-12, GlutaPlus

G4562-500ML

500 mL

 


Product Description/Introduction

Hams F-12 (Hams F-12 Nutrient Mixture) is based on Ham's F-10 nutrient mix and was originally designed for serum-free culture of CHO cells. Ham's F-12 is often used as a basal culture medium for serum-free cultures and is particularly suitable for single cell cultures and clonogenic cultures at low serum levels. It is also widely used in the culture of tumor cells and primary cells by adding serum, such as rat hepatocytes, rat prostate epithelial cells, chondrocytes, rat myogenic cells, chicken embryonic cells, etc. In addition, Ham's F-12 is mixed with DMEM in equal volume to obtain a more nutrient-rich DMEM/F-12 medium.

This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Need to add serum or serum-free additives depending on the cell type.

This product is filtered and sterilized by 0.1 μm filter membrane, pH 7.0-7.4, contains 10 mM D-Glucose, 1.0 mM L-Alanyl-L-Glutamine, 1.0 mM sodium pyruvate and phenol red indicator, without HEPES.

 


Storage and Shipping Conditions

Ship at room temperature; Store away from light at 2-8, valid for 12 months.

 


Note

1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.

2. For best results, do not freeze and thaw repeatedly.

3. For your safety and health, please wear safety glasses, gloves and protective clothing.

 

For Research Use Only!

G4562 Hams F-12 Formula

Components

Concentration


Components

Concentration

mg/L

mM

mg/L

mM

Amino acids




Inorganic salts



Glycine

7.507

0.1


Potassium Chloride (KCl)

223.6

2.98

L-Alanine

8.90

0.1


Sodium Bicarbonate (NaHCO3)

1176

14

L-Arginine hydrochloride

210.66

1.0


Sodium Chloride (NaCl)

7599

131.02

L-Asparagine-H2O

15.02

0.1


Dibasic sodium phosphate (Na2HPO4) (anhyd.)

141.96

1.0

L-Aspartic Acid

13.31

0.1


Calcium Chloride (CaCl2)(anhyd.)

33.22

0.30

L-Cysteine hydrochloride-H2O

35.05

0.20


Copper Sulfate Pentahydrate (CuSO4·5H2O

0.0025

0.00001

L-Glutamic Acid

14.71

0.1


Iron Sulfate Heptahydrate (FeSO4·5H2O

0.834

0.003

L-Alanyl-L-Glutamine

217.2

1.0


Magnesium Chloride (MgCl2)(anhyd.)

57.35

0.60

L-Histidine hydrochloride-H2O

20.96

0.1


Zinc Sulfate Heptahydrate (ZnSO4·7H2O

0.862

0.003

L-Isoleucine

4.005

0.031


Other components



L-Leucine

13.12

0.1


D-Glucose (Dextrose)

1802.0

10.01

L-Lysine hydrochloride

36.5

0.199


Phenol red

1.13

0.00319

L-Methionine

4.5

0.03


Sodium pyruvate

110

1.0

L-Phenylalanine

5.0

0.03


Hypoxanthine

4.083

0.03

L-Proline

34.5

0.3


Linoleic Acid

0.084

0.0003

L-Serine

10.5

0.1


Alphalipoic Acid

0.210

0.00102

L-Threonine

11.9

0.10


Diaminobutane dihydrochloride

0.161

0.001

L-Tryptophan

2.0

0.01


Thymidine

0.701

0.00289

L-Tyrosine Disodium Salt

7.8

0.0298





L-Valine

11.7

0.1





Vitamins







Biotin

0.0073

0.0000299





Choline chloride

14

0.1





D-Calcium pantothenate

0.50

 

0.001048





Folic Acid

1.30

0.002948





Niacinamide

0.04

0.000295





Pyridoxine hydrochloride

0.06

0.000291





Riboflavin

0.04

0.000098





Thiamine hydrochloride

0.30

0.000890





Vitamin B12

1.40

0.001033





i-Inositol

18

0.1





 

 

 

 


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