Recombinant Mouse LF Protein (His-tag)

Gene Name Synonym                

LF, TRFL, LTF, GIG12

Species                

Mouse

Accession                

P08071

Source                

HEK293 cells

SPECIFICATIONS                

Amino acid sequence                

Ala20-Arg707 is expressed with a 10×His tag at the C terminus

Molecular Weight                

77.2 kDa

Purity                

>95.0% as determined by SDS-PAGE

Endotoxin                

<5 EU mg^-1                

Formulation                

Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution                

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE                

Stability and Storage                

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.                

BACKGROUND                

Lactoferrin (LF) is a multifunctional protein occurring in many biological secretions including milk. It possesses iron binding/transferring, antibacterial, antiviral, antifungal, anti-inflammatory
and anti-carcinogenic properties. LF is primarily extracted from bovine milk and it is subsequently added into many commercial products such as nutritional supplements, infant formula, cosmetics and toothpaste. Lactoferrin interacts with DNA and RNA, polysaccharides and heparin, and shows some of its biological functions in complexes with these ligands. Although the mechanism underlying the action of Lf in bone cells is still not fully elucidated, it has been shown that its mode of action leading to the survival of osteoblasts is complemented by its mitogenic effect. LF is sensitive to denaturation induced by temperature and other physicochemical stresses. The bioavailability of LF in vivo is poor.

Data                

SDS-PAGE                

Bioactivity-Cell based assay                

Bioactivity-ELISA                

Recombinant Mouse LF Protein was determined by SDS-PAGE with Coomassie Blue.

Measured by its ability to induce TNF-α secretion by THP-1 cells. The ED50 for this effect is 11 μg/mL.

Immobilized Mouse LF, His Tag at 5 μg/mL (100 μL/well) can bind Anti-Lactoferrin with a linear range of 0.019-0.312 ng/mL.