Recombinant Mouse PDGFD Protein (His-tag)

Gene Name Synonym

PDGF-DD, Platelet-derived growth factor D, SCDGFB

Species

Mouse

Accession

Q925I7

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Thr24-Arg370 is expressed with a 10×His tag at the C terminus

Molecular Weight

40.2 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

PDGF-DD is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines, seven of which are found in this factor. This gene product only forms homodimers and, therefore, does not dimerize with the other three family members. It differs from alpha and beta members of this family in having an unusual N-terminal domain, the CUB domain. PDGF-DD specifically binds to and activates its cognate receptor PDGFR-β, and participates in regulating cancer proliferation, transformation, and invasion through activating multiple downstream oncogenic pathways, resulting in tumor development and progression.

Data

SDS-PAGE

Bioactivity

Active Recombinant Mouse PDGFD Protein was determined by SDS-PAGE with Coomassie Blue.

Measured in a cell proliferation assay using Balb/C 3T3 A31 mouse embryonic fibroblast cells. The ED50 for this effect is 2-5 μg/mL.