Recombinant Rat TGF-beta1 Protein (His-tag)

Gene Name Synonym

EDLAP, TGF-beta 1, TGFB1, DPD1, TGF-beta-1, TGFB

Species

Rat

Accession

P17246

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Leu30-Ser390 is expressed with a 10×His tag at the C terminus

Molecular Weight

41.3 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

Transforming growth factor beta 1 ( TGFB1) is also known as TGF-β1, CED, DPD1, TGFB. is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation and apoptosis. The TGFB1 protein helps control the growth and division (proliferation) of cells, the process by which cells mature to carry out specific functions (differentiation), cell movement (motility), and the self-destruction of cells (apoptosis). The TGFB1 protein is found throughout the body and plays a role in development before birth, the formation of blood vessels, the regulation of muscle tissue and body fat development, wound healing, and immune system function. TGFB1 is particularly abundant in tissues that make up the skeleton, where it helps regulate bone growth, and in the intricate lattice that forms in the spaces between cells (the extracellular matrix). Within cells, this protein is turned off (inactive) until it receives a chemical signal to become active.

Data

SDS-PAGE

Bioactivity

Active Recombinant Rat TGF-beta1 Protein was determined by SDS-PAGE with Coomassie Blue.The apparent molecular mass of the recombinant protein is approximately 55, 38 and 16 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Measured by its ability to induce cell proliferation of MCF-7 human breast cancer cells. The ED50 for this effect is 0.1-0.5 μg/mL.