Recombinant Human CD4 Protein (His-tag)

Gene Name Synonym

CD4, LEU3

Species

Human

Accession

P01730

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Lys26-Pro396 is expressed with a 10×His tag at the N terminus

Molecular Weight

41.5 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

CD4 is a monomeric glycoprotein expressed on approximately 60% of peripheral blood T lymphocytes. CD4 (T4), a glycoprotein expressed largely on the surface of cells in the immune system, serves as the receptor for the human immunodeficiency virus, HIV. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. CD4 is a co-receptor that assists the T cell receptor (TCR) to activate its T cell following an interaction with an antigen-presenting cell. The CD4 molecule plays an important role in the development of CD4+T lymphocytes and it also acts as a coreceptor to enhance responses mediated via the TCR and also subserves as the receptor for the human immunodeficiency viruses.

Data

SDS-PAGE

Bioactivity

Testing in progress.

Recombinant Human CD4 Protein was determined by SDS-PAGE with Coomassie Blue, showing band at 42-55 kDa.