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หมวดหมู่ : 3. Protein & Enzyme , Recombinant protein , Servicebio ,
แบรนด์ : Servicebio
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DESCRIPTION | ||
Gene Name Synonym | PDL1, CD274, B7H1, PDCD1L1, PDCD1LG1 | |
Species | Human | |
Accession | Q9NZQ7 | |
Source | HEK293 cells | |
SPECIFICATIONS | ||
Amino acid sequence | >sp|Q9NZQ7|19-238 is expressed with a 10×His tag at the N terminus | |
Molecular Weight | 25.2 kDa | |
Purity | >95.0% as determined by SDS-PAGE | |
Endotoxin | <5 EU mg-1 | |
Formulation | Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. | |
Reconstitution | Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation. | |
STORAGE | ||
Stability and Storage | 12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. | |
BACKGROUND | ||
Data | ||
SDS-PAGE | Bioactivity | |
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Active Recombinant Human PDL1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 30-40 kDa. | Measured by its binding ability in a functional ELISA. Immobilized human PDL1 at 3 μg/ml (100 μl /well) can bind biotinylated human PD1 with a linear range of 0.039-0.652 μg/mL. | |
