Recombinant Human CD31 Protein (His-tag)

Gene Name Synonym

CD31, PECAM1

Species

Human

Accession

P16284

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Gln28-Lys601 is expressed with a 10×His tag at the N terminus

Molecular Weight

64.5 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

CD31 is a transhomophilic tyrosine-based inhibitory motif receptor and is expressed by both dendritic cells (DCs) and T lymphocytes.CD31 is the most abundant constitutive co-signalling receptor glycoprotein on endothelial cells.CD31 also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1), is the only known member of the CAM family on platelets. CD31 protein mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin αvβ3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development.

Data

SDS-PAGE

Bioactivity

Active Recombinant Human CD31 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 65-90 kDa.

Measured by its binding ability in a functional ELISA. Immobilized human CD31 at 3 μg/ml (100 μl /well) can bind biotinylated human CTNNB1 with a linear range of 0.06-0.5 μg/mL.