Recombinant Mouse CD68 Protein (His-tag)

Gene Name Synonym

CD68, GP110, LAMP4, SCARD1

Species

Mouse

Accession

P31996

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Asp21-Ser291 is expressed with a 10×His tag at the N terminus

Molecular Weight

28.8 kDa（The protein migrates as 40~60 kDa under reducing condition (SDS-PAGE) due to glycosylation）

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

CD68 is a heavily glycosylated glycoprotein that is highly expressed in macrophages and other mononuclear phagocytes. Macrosialin, also known as CD68 and Gp11, is a single-pass type I membrane protein. However, a number of studies have shown that CD68 antibodies react with other hematopoietic and non-hematopoietic cell types, suggesting that CD68 may not be a macrophage-specific antigen. CD68 alone or in combination with other cell markers of tumor-associated macrophages showed a good predictive value as a prognostic marker of survival in cancer patients. CD68 is mainly located in the endosomal/lysosomal compartment but can rapidly shuttle to the cell surface.

Data

SDS-PAGE

Bioactivity

Active Recombinant Mouse CD68 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 40-65 kDa.

Immobilized Mouse CD68, His Tag at 5 μg/mL (100 μL/well) can bind Anti-CD68 with a linear range of 0.03-1.25 μg/mL.