Fast Pfu DNA Polymerase, 200 U

Product Information

Product Description/Introduction

Fast Pfu DNA polymerase is a modified high-fidelity Pfu DNA polymerase that incorporates a single-stranded binding protein. The amplification ability is strong, the amplification speed is fast, the elongation speed can reach 5-15s/kb, with high fidelity, high specificity, with 5'-3' polymerase activity and 3'-5' exonuclease activity, and the amplified product is flat terminal. It is suitable for PCR amplification of conventional PCR, colony PCR, complex template and high GC content template. DNA fragments up to 14kb can be amplified.

Source: Derived from Pyrococcus furiosus, recombinant expression by Escherichia coli.

Enzyme activity definition: Using activated salmon sperm DNA as template primer, the enzyme required for incorporation of 10nmol deoxynucleotides into acid insoluble substances was defined as an enzyme activity unit within 30min at 74.

Enzyme storage buffer:10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% Tween 20, 0.5% NP-40, 50% Glycerol, pH 8.0.

Storage and Shipping Conditions

wetice transport; Store at -20 for 12 months.

Product Content

Assay Protocol / Procedures

1. This product is mainly sold in the form of Fast Pfu DNA polymerase raw materials, and customers need to optimize the reaction buffer and reaction system according to their own needs. G3305 is recommended for purchase of PCR premixes.

Note

1. Enzyme products should be placed in an ice box or ice bath when used, and should be stored at -20 immediately after use, it is recommended to store in separate packages.

2. For your safty and health,please wear safety glasses, gloves, or protective clothing. 

For Research Use Only!

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