SKU : G3414-10KU
หมวดหมู่ : Biochemicals , 1. Chemical and Reagents , Buffers , Servicebio ,
แบรนด์ : Servicebio
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Product Information
Product Name | Cat. No. | Spec. |
RNase Inhibitor, Porcine | G3414-10KU | 10 KU |
Product Introduction
Product Description: | The RNase Inhibitor (Ribonuclease Inhibitor） inhibits the activity of RNases A, B and C by binding them in a noncompetitive mode at a 1:1 ratio. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. The performance of Ribonuclease inhibitors from porcine and human is similar. This product exerts its inhibitory effect at pH 5-8, showing the strongest inhibitory effect at pH 7-8. A final concentration of 1mM DTT at least in the storage solution is required to maintain the activity of RNase inhibitor. |
Applications: | l RT-PCR l cDNA synthesis · In vitro transcription/translation · Enzymatic RNA labeling reaction l Other applications where the integrity of RNA is important |
Source: | An E. coli strain that carries the Ribonuclease Inhibitor gene from porcine. |
Purity: | 95% by SDS-PAGE |
Concentration: | 40 U/μL |
Definition of Activity Unit: | One unit is defined as the amount of RNase Inhibitor required to inhibit the activity of 5ng of RNase A by 50%. |
Storage (Dilution) Buffer: | 20 mM Tris-HCl, 50 mM NaCl, 5 mM DTT, 50% (v/v) glycerol，pH 7.5. |
Inactivation or inhibition | l Inhibitors: common denaturants (SDS, urea and all oxidizing reagents (p-chloromercuribenzoate, dissolved oxygen, ions in their higher oxidation states) strongly inhibit RNase Inhibitor and release the RNase bound. l Inactivated by heating at 75°C for 10 min. |
Storage Conditions: | Store at 20°C up to 12 months. |
Product Contents
Component Number | Component | G3414-10KU |
G3414-1 | RNase Inhibitor, Porcine | 250 μL |
Assay Protocol / Procedures
1. The recommended concentration of RNase Inhibitor in a reaction is 1-2 unit/μL. During assembly of a reaction (eg. in vitro transcription, cDNA synthesis, in vitro translation), RNase Inhibitor should be added before other components that are a possible source of RNase contamination.
Note
l Enzymes should be placed on ice when used, and stored at -20 immediately after use. It is recommended to store separately.
1. For your safety and health, please wear safety glasses, gloves, or protective clothing.
For Research Use Only!
